Engineering mesenchymal stem cell spheroids by incorporation of mechanoregulator microparticles

Journal of the Mechanical Behavior of Biomedical Materials
Fatemeh AbbasiMohamadreza Baghaban Eslaminejad

Abstract

Mechanical forces throughout human mesenchymal stem cell (hMSC) spheroids (mesenspheres) play a predominant role in determining cellular functions of cell growth, proliferation, and differentiation through mechanotransductional mechanisms. Here, we introduce microparticle (MP) incorporation as a mechanical intervention method to alter tensional homeostasis of the mesensphere and explore MSC differentiation in response to MP stiffness. The microparticulate mechanoregulators with different elastic modulus (34 kPa, 0.6 MPa, and 2.2 MPa) were prepared by controlled crosslinking cell-sized microdroplets of polydimethylsiloxane (PDMS). Preparation of MP-MSC composite spheroids enabled us to study the possible effects of MPs through experimental and computational assays. Our results showed that MP incorporation selectively primed MSCs toward osteogenesis, yet hindered adipogenesis. Interestingly, this behavior depended on MP mechanics, as the spheroids that contained MPs with intermediate stiffness behaved similar to control MP-free mesenspheres with more tendencies toward chondrogenesis. However, by using the soft or stiff MPs, the MP-mesenspheres significantly showed signs of osteogenesis. This could be explained by the complex of f...Continue Reading

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Citations

Aug 1, 2020·Advanced Healthcare Materials·Se-Jeong KimHeungsoo Shin
Jul 25, 2020·Advanced Drug Delivery Reviews·Samad AhadianAli Khademhosseini
Jul 9, 2021·Journal of Materials Science. Materials in Medicine·Mehdi MoradkhaniBahram Ahmadian

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