Engineering metabolic pathways in Escherichia coli for constructing a "microbial chassis" for biochemical production

Bioresource Technology
Takuya MatsumotoAkihiko Kondo

Abstract

The present work reviews literature describing the re-design of the metabolic pathways of a microbial host using sophisticated genetic tools, yielding strains for producing value-added chemicals including fuels, building-block chemicals, pharmaceuticals, and derivatives. This work employed Escherichia coli, a well-studied microorganism that has been successfully engineered to produce various chemicals. E. coli has several advantages compared with other microorganisms, including robustness, and handling. To achieve efficient productivities of target compounds, an engineered E. coli should accumulate metabolic precursors of target compounds. Multiple researchers have reported the use of pathway engineering to generate strains capable of accumulating various metabolic precursors, including pyruvate, acetyl-CoA, malonyl-CoA, mevalonate and shikimate. The aim of this review provides a promising guideline for designing E. coli strains capable of producing a variety of useful chemicals. Herein, the present work reviews their common and unique strategies, treating metabolically engineered E. coli as a "microbial chassis".

Citations

Nov 18, 2017·The Biochemical Journal·Selçuk AslanArren Bar-Even
Jan 4, 2020·The Journal of Microbiology·Jun RenDokyun Na
Sep 24, 2019·Journal of Chromatography. a·Ricardo FradiqueM Raquel Aires-Barros
Feb 8, 2020·Trends in Biotechnology·Xianhao XuLong Liu
Oct 24, 2018·Chemical Reviews·Nuno R CandeiasSvilen P Simeonov
Nov 12, 2021·Applied Microbiology and Biotechnology·Eldin KurpejovićBerna Sariyar Akbulut

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