Engineering Multi-Walled Carbon Nanotube Therapeutic Bionanofluids to Selectively Target Papillary Thyroid Cancer Cells

PloS One
Idit DotanMark A Trifiro

Abstract

The incidence of papillary thyroid carcinoma (PTC) has risen steadily over the past few decades as well as the recurrence rates. It has been proposed that targeted ablative physical therapy could be a therapeutic modality in thyroid cancer. Targeted bio-affinity functionalized multi-walled carbon nanotubes (BioNanofluid) act locally, to efficiently convert external light energy to heat thereby specifically killing cancer cells. This may represent a promising new cancer therapeutic modality, advancing beyond conventional laser ablation and other nanoparticle approaches. Thyroid Stimulating Hormone Receptor (TSHR) was selected as a target for PTC cells, due to its wide expression. Either TSHR antibodies or Thyrogen or purified TSH (Thyrotropin) were chemically conjugated to our functionalized Bionanofluid. A diode laser system (532 nm) was used to illuminate a PTC cell line for set exposure times. Cell death was assessed using Trypan Blue staining. TSHR-targeted BioNanofluids were capable of selectively ablating BCPAP, a TSHR-positive PTC cell line, while not TSHR-null NSC-34 cells. We determined that a 2:1 BCPAP cell:α-TSHR-BioNanofluid conjugate ratio and a 30 second laser exposure killed approximately 60% of the BCPAP cells, w...Continue Reading

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Citations

Mar 30, 2017·Endocrine-related Cancer·Christopher W RoweRoger Smith
May 10, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Mojgan SheikhpourAlibakhsh Kasaeian
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Aug 18, 2020·Nanotechnology, Science and Applications·Mojgan SheikhpourZahra Taherian
Jun 11, 2021·Frontiers in Chemistry·Gurpal SinghRavi Pratap Barnwal

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Methods Mentioned

BETA
dissection
Scanning electron microscopy

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