Engineering of a near-infrared fluorescent probe for real-time simultaneous visualization of intracellular hypoxia and induced mitophagy

Chemical Science
Yongchao LiuXiao-Bing Zhang

Abstract

Mitophagy induced by hypoxia plays an important role in regulating cellular homeostasis via the removal of dysfunctional mitochondria in the lysosomal degradation pathway, which results in physiological changes in the mitochondria, such as the pH, polarity and viscosity. However, the lack of an effective method for imaging of both the hypoxic microenvironment and the resulting variable mitochondria limits the visualization of hypoxia-induced mitophagy. Based on the specific mitochondrial pH changes during the hypoxia-induced mitophagy process, we have reported a near-infrared fluorescent probe (NIR-HMA) for real-time simultaneous visualization of the hypoxic microenvironment and the subsequent mitophagy process in live cells. NIR-HMA selectively accumulated in the hypoxic mitochondria in the NIR-MAO form, emitting at 710 nm, and then transformed into NIR-MAOH, emitting at 675 nm, in the acidified mitochondria-containing autolysosomes. Importantly, by smartly tethering the hypoxia-responsive group to the hydroxyl group of the NIR-fluorochrome, which shows ratiometric pH changes, NIR-HMA can differentiate between different levels of the hypoxic microenvironment and mitophagy. Furthermore, using NIR-HMA, we could track the complet...Continue Reading

References

Sep 1, 1996·Molecular and Cellular Biology·J A ForsytheG L Semenza
Mar 21, 2002·Nature Reviews. Cancer·Adrian L Harris
Sep 9, 2005·Nature Reviews. Cancer·Yasuko KondoSeiji Kondo
Oct 22, 2008·Oncogene·J-P PursiheimoP M Jaakkola
Dec 22, 2009·Current Opinion in Cell Biology·Nathalie M Mazure, Jacques Pouysségur
Feb 11, 2010·Cell·Noboru MizushimaBeth Levine
Dec 24, 2010·Nature Reviews. Molecular Cell Biology·Richard J Youle, Derek P Narendra
May 16, 2014·Nature Reviews. Cancer·Daniele M GilkesDenis Wirtz
Aug 27, 2014·Journal of the American Chemical Society·Min Hee LeeJong Seung Kim
Oct 7, 2014·Chemical Society Reviews·Min Hee LeeJonathan L Sessler
Jan 21, 2015·Biochimica Et Biophysica Acta·Tomotake KankiShun-ichi Yamashita
Apr 30, 2015·Journal of the American Chemical Society·Yuhao LiFuyou Li
Jun 18, 2016·Chemical Reviews·Wen SunXiaojun Peng
Aug 31, 2016·Journal of the American Chemical Society·Ying LiuWeihong Tan
Sep 7, 2016·Oncogene·E E MowersK F Macleod
Oct 12, 2017·Chemical Society Reviews·Di WuTony D James
Jan 10, 2018·Angewandte Chemie·Qingqing MiaoKanyi Pu
Jan 1, 2016·Chemical Science·Avik Ranjan SarkarHwan Myung Kim

❮ Previous
Next ❯

Citations

Dec 7, 2018·Chemical Society Reviews·Junji ZhangHe Tian
Mar 26, 2019·Chemical Communications : Chem Comm·Hongbo ChenYalin Tang
Oct 10, 2019·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Subhajit ChakrabortySaptarshi Mukherjee
Jan 30, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaodong WangChuan Dong
Sep 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chenggong MaBaoxing Shen
Oct 15, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fanpeng KongBo Tang
Nov 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaolin LiChusen Huang
Mar 9, 2020·Science China. Life Sciences·Fengfeng XueHangrong Chen
Jul 16, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sophie BouMayeul Collot
Nov 27, 2020·Advanced Healthcare Materials·Miffy Hok Yan ChengGang Zheng
Jan 8, 2021·Organic & Biomolecular Chemistry·Madhu RameshThimmaiah Govindaraju
Nov 30, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sébastien JenniAnthony Romieu
Nov 7, 2020·Chemical Society Reviews·Xuechen LiWeiying Lin
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yunnan YanHaiying Liu
May 14, 2021·Chemical Communications : Chem Comm·Zun-Pan SheChun-Yan Li
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shenggang WangXiangming Guan
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Feng LiuZhihui Li
Aug 14, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yi-Lin MuXian Zhang
Dec 20, 2018·Analytical Chemistry·Dandan MaRonghua Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.