Engineering of a silica encapsulation platform for hydrocarbon degradation using Pseudomonas sp. NCIB 9816-4

Biotechnology and Bioengineering
Jonathan K SakkosAlptekin Aksan

Abstract

Industrial application of encapsulated bacteria for biodegradation of hydrocarbons in water requires mechanically stable materials. A silica gel encapsulation method was optimized for Pseudomonas sp. NCIB 9816-4, a bacterium that degrades more than 100 aromatic hydrocarbons. The design process focused on three aspects: (i) mechanical property enhancement; (ii) gel cytocompatibility; and (iii) reduction of the diffusion barrier in the gel. Mechanical testing indicated that the compressive strength at failure (σf ) and elastic modulus (E) changed linearly with the amount of silicon alkoxide used in the gel composition. Measurement of naphthalene biodegradation by encapsulated cells indicated that the gel maintained cytocompatibility at lower levels of alkoxide. However, significant loss in activity was observed due to methanol formation during hydrolysis at high alkoxide concentrations, as measured by FTIR spectroscopy. The silica gel with the highest amount of alkoxide (without toxicity from methanol) had a biodegradation rate of 285 ± 42 nmol/L-s, σf  = 652 ± 88 kPa, and E = 15.8 ± 2.0 MPa. Biodegradation was sustained for 1 month before it dropped below 20% of the initial rate. In order to improve the diffusion through the gel...Continue Reading

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Citations

Jun 7, 2016·Scientific Reports·Baris R MutluAlptekin Aksan
Jun 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohammed Umar MustaphaMohamed Yunus Abd Shukor
Apr 16, 2019·Frontiers in Microbiology·Michael SchwabMikael Elias
Jul 19, 2017·ACS Applied Materials & Interfaces·Jonathan K SakkosAlptekin Aksan

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