Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells

Scientific Reports
Giulia CerinoAnna Marsano

Abstract

In vitro recapitulation of an organotypic stromal environment, enabling efficient angiogenesis, is crucial to investigate and possibly improve vascularization in regenerative medicine. Our study aims at engineering the complexity of a vascular milieu including multiple cell-types, a stromal extracellular matrix (ECM), and molecular signals. For this purpose, the human adipose stromal vascular fraction (SVF), composed of a heterogeneous mix of pericytes, endothelial/stromal progenitor cells, was cultured under direct perfusion flow on three-dimensional (3D) collagen scaffolds. Perfusion culture of SVF-cells reproducibly promoted in vitro the early formation of a capillary-like network, embedded within an ECM backbone, and the release of numerous pro-angiogenic factors. Compared to static cultures, perfusion-based engineered constructs were more rapidly vascularized and supported a superior survival of delivered cells upon in vivo ectopic implantation. This was likely mediated by pericytes, whose number was significantly higher (4.5-fold) under perfusion and whose targeted depletion resulted in lower efficiency of vascularization, with an increased host foreign body reaction. 3D-perfusion culture of SVF-cells leads to the enginee...Continue Reading

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Citations

Dec 12, 2019·Journal of Tissue Engineering and Regenerative Medicine·Mohammad Ali NilforoushzadehMehdi Nouradini
Dec 12, 2018·Frontiers in Physiology·Yulia A PaninaAlla B Salmina
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Thomas SpäterMatthias W Laschke
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Anastasiya A GorkunIrina N Saburina

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
profiler
flow cytometry
Assay
ELISA

Software Mentioned

CellSens
Flowjo
GraphPad
ImageJ
GraphPad Prism

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