Engineering of bacterial exotoxins for highly efficient and receptor-specific intracellular delivery of diverse cargos

Biotechnology and Bioengineering
Jeong-Hyun RyouHak-Sung Kim

Abstract

The intracellular delivery of proteins with high efficiency in a receptor-specific manner is of great significance in molecular medicine and biotechnology, but remains a challenge. Herein, we present the development of a highly efficient and receptor-specific delivery platform for protein cargos by combining the receptor binding domain of Escherichia coli Shiga-like toxin and the translocation domain of Pseudomonas aeruginosa exotoxin A. We demonstrated the utility and efficiency of the delivery platform by showing a cytosolic delivery of diverse proteins both in vitro and in vivo in a receptor-specific manner. In particular, the delivery system was shown to be effective for targeting an intracellular protein and consequently suppressing the tumor growth in xenograft mice. The present platform can be widely used for intracellular delivery of diverse functional macromolecules with high efficiency in a receptor-specific manner. Biotechnol. Bioeng. 2016;113: 1639-1646. © 2016 Wiley Periodicals, Inc.

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Citations

Aug 25, 2018·Scientific Reports·Bruce R Lichtenstein, Birte Höcker
Jun 3, 2021·International Journal of Molecular Sciences·Olga ShilovaSergey Deyev
Jun 3, 2021·Toxins·Clifford Lingwood
Apr 27, 2019·ACS Chemical Biology·Nadine Eliane SchmitOliver Hantschel
Jul 28, 2018·Chemical Reviews·Martin P StewartKlavs F Jensen

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