Engineering of baker's yeasts, E. coli and Bacillus hosts for the production of Bacillus subtilis Lipase A

Biotechnology and Bioengineering
Marta SánchezP Diaz

Abstract

Lipases are versatile biocatalists showing multiple applications in a wide range of biotechnological processes. The gene lipA coding for Lipase A from Bacillus subtilis was isolated by PCR amplification, cloned and expressed in Escherichia coli, Saccharomyces cerevisiae and Bacillus subtilis strains, using pBR322, YEplac112 and pUB110-derived vectors, respectively. Lipase activity analysis of the recombinant strains showed that the gene can be properly expressed in all hosts assayed, this being the first time a lipase from bacterial origin can be expressed in baker's S. cerevisiae strains. An important increase of lipase production was obtained in heterologous hosts with respect to that of parental strains, indicating that the described systems can represent a useful tool to enhance productivity of the enzyme for biotechnological applications, including the use of the lipase in bread making, or as a technological additive.

References

Dec 1, 1986·Mutation Research·G DasF Sherman
Jan 1, 1987·Applied and Environmental Microbiology·G Kouker, K E Jaeger
Sep 11, 1985·Nucleic Acids Research·L Sarokin, M Carlson
Feb 1, 1986·Human Genetics·S L Sherman, G R Sutherland
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Sep 1, 1994·FEMS Microbiology Reviews·K E JaegerO Misset
May 1, 1994·Applied and Environmental Microbiology·V DartoisA Baulard
May 20, 1994·Journal of Molecular Biology·S RansacB W Dijkstra
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 23, 1998·Trends in Biotechnology·K E Jaeger, M T Reetz
Sep 24, 1999·The Biochemical Journal·J L Arpigny, K E Jaeger
Oct 19, 1999·BioTechniques·P DiazF I Javier Pastor

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Citations

May 17, 2012·Applied Microbiology and Biotechnology·Jingnan LuAnthony J Sinskey
Jun 4, 2008·Journal of Industrial Microbiology & Biotechnology·Serpil Takaç, Başak Marul
Apr 7, 2005·Applied and Environmental Microbiology·Helga WestersWim J Quax
Mar 31, 2004·Canadian Journal of Microbiology·Marcus SchallmeyOwen P Ward
Oct 10, 2002·Yeast
Oct 30, 2018·Recent Patents on Biotechnology·Naveen PatelUmesh Mishra

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