Engineering of extensor tendon complex by an ex vivo approach

Biomaterials
Bin WangYilin Cao

Abstract

Engineering of extensor tendon complex remains an unexplored area in tendon engineering research. In addition, less is known about the mechanism of mechanical loading in human tendon development and maturation. In the current study, an ex vivo approach was developed to investigate these issues. Human fetal extensor tenocytes were isolated, expanded and seeded on polyglycolic acid (PGA) fibers that formed a scaffold with a shape mimicking human extensor tendon complex. After in vitro culture for 6 weeks, 7 cell-scaffold constructs were further in vitro cultured with dynamic mechanical loading for another 6 weeks in a bioreactor. The other 14 constructs were in vivo implanted subcutaneously to nude mice for another 14 weeks. Seven of them were implanted without loading, whereas the other 7 were sutured to mouse fascia and animal movement provided a natural dynamic loading in vivo. The results demonstrated that human fetal cells could form an extensor tendon complex structure in vitro and become further matured in vivo by mechanical stimulation. In contrast to in vitro loaded and in vivo non-loaded tendons, in vivo loaded tendons exhibited bigger tissue volume, better aligned collagen fibers, more mature collagen fibril structure ...Continue Reading

References

Sep 25, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R G YoungD J Fink
Aug 6, 1999·Tissue Engineering·H A AwadA I Caplan
Dec 29, 2000·Plastic and Reconstructive Surgery·W B RockwellB A Byrne
Apr 23, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hani A AwadDavid L Butler
Oct 28, 2003·The Journal of Physiology·Oksana KostyukRobert A Brown
Nov 25, 2003·Tissue Engineering·Joanne GarvinAlbert J Banes
Sep 3, 2005·Biochemical and Biophysical Research Communications·Hazel R C ScreenDavid A Lee
Nov 29, 2005·Journal of Bioscience and Bioengineering·Qi-Wei WangYing-Jie Piao
Mar 7, 2006·The Journal of Hand Surgery·Alphonsus K S Chong, James Chang
Apr 6, 2006·The Journal of Clinical Investigation·Andrea HoffmannDan Gazit
Dec 16, 2006·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Zhonggang FengTakao Nakamura
Jan 4, 2007·The Journal of Bone and Joint Surgery. American Volume·Alphonsus K S ChongBeng Hai Lim
Jan 11, 2007·Tissue Engineering·Michelle Hairfield-SteinPaul Kosnik
May 8, 2007·The Journal of Hand Surgery·Gil S KrygerJames Chang

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Citations

Nov 23, 2011·Journal of Materials Science. Materials in Medicine·A EnglishD I Zeugolis
Jun 18, 2011·Frontiers of Medicine·Hengyun SunYilin Cao
Nov 27, 2008·Journal of the Royal Society, Interface·Mark R PlaczekAthanasios Mantalaris
May 29, 2010·Journal of Biomaterials Science. Polymer Edition·Jongman LeeSoo Hyun Kim
Dec 17, 2014·Advanced Drug Delivery Reviews·A J LomasD I Zeugolis
Feb 23, 2010·Biomaterials·Liang XuYilin Cao
Aug 18, 2009·Journal of Tissue Engineering and Regenerative Medicine·Hallie E BrinkSteven B Nicoll
Apr 22, 2016·Journal of Tissue Engineering and Regenerative Medicine·Yongkang JiangWei Liu
Sep 29, 2019·Stem Cells International·Marta BottagisioArianna B Lovati
Apr 10, 2019·Annals of Biomedical Engineering·Giuseppe TalòArianna B Lovati
Oct 9, 2020·Stem Cells International·Renwang ShengJialin Chen
Apr 4, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sandra Ruiz-AlonsoJose Luis Pedraz
May 4, 2021·Frontiers in Cell and Developmental Biology·Yuange LiShen Liu

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