PMID: 1309953Jan 15, 1992Paper

Engineering of glucose-stimulated insulin secretion and biosynthesis in non-islet cells

Proceedings of the National Academy of Sciences of the United States of America
S D HughesC B Newgard


The high-capacity glucose transporter known as GLUT-2 and the glucose phosphorylating enzyme glucokinase are thought to be key components of the "glucose-sensing apparatus" that regulates insulin release from the beta cells of the islets of Langerhans in response to changes in external glucose concentration. AtT-20ins cells are derived from anterior pituitary cells and are like beta cells in that they express glucokinase and have been engineered to secrete correctly processed insulin in response to analogs of cAMP, but, unlike beta cells, they fail to respond to glucose and lack GLUT-2 expression. Herein we demonstrate that stable transfection of AtT-20ins cells with the GLUT-2 cDNA confers glucose-stimulated insulin secretion and glucose regulation of insulin biosynthesis and also results in glucose potentiation of the secretory response to non-glucose secretagogues. This work represents a first step toward creation of a genetically engineered "artificial beta cell."


Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L ChenR H Unger
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B ThorensS Bonner-Weir
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L OrciR H Unger
Mar 1, 1990·Diabetes Care·B ThorensH F Lodish
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D J GrossL Villa-Komaroff
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C B NewgardR J Fletterick
Jan 1, 1986·Diabetes/metabolism Reviews·M D Meglasson, F M Matschinsky
Jan 1, 1986·Annual Review of Medicine·P E Lacy, D W Scharp
Jan 1, 1985·Annual Review of Immunology·A A RossiniA A Like
Jan 1, 1963·Advances in Enzyme Regulation·S WEINHOUSEH P MORRIS
Jun 21, 2008·World Journal of Gastroenterology : WJG·Mercedes Susan MandellMichael Zimmerman

❮ Previous
Next ❯


Dec 1, 1992·Diabetologia·D F Steiner, D E James
Jan 1, 1993·Applied Biochemistry and Biotechnology·D C Cameron, I T Tong
Apr 11, 2003·Regulatory Peptides·Hideki KizawaMasahiko Fujino
Dec 7, 2000·Diabetes Research and Clinical Practice·J H NamK B Huh
Mar 31, 2000·Diabetes Research and Clinical Practice·M F McInerneyC A Janeway
Nov 13, 2008·Cytotechnology·Patrick GammellMartin Clynes
Feb 15, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jaeseok HanHee-Sook Jun
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M StoffelS Lesage
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L R InmanJ H Johnson
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M A LipesA M Davalli
Jun 21, 2012·Tissue Engineering. Part C, Methods·Lisa Samuelson, David A Gerber
Feb 1, 1993·European Journal of Clinical Investigation·B H Wolffenbuttel, T W Van Haeften
Nov 1, 1995·The Journal of Clinical Investigation·A De VosF Schuit
May 25, 2002·In Vitro Cellular & Developmental Biology. Animal·Lorraine O'DriscollMartin Clynes
Dec 6, 2002·Drugs·Nikolai PetrovskyDesmond A Schatz
Jun 22, 2005·Treatments in Endocrinology·Daniel M RidgwayRachel M Kimber
Nov 6, 2001·Revista clínica española·G BernáB Soria
Jul 6, 2010·Metabolism: Clinical and Experimental·Ke ChenHiroshi Tokumitsu
Oct 7, 2003·Annual Review of Biomedical Engineering·Martin L Yarmush, Scott Banta
Jun 1, 1997·The Journal of Surgical Research·H TaniguchiH Nakauchi
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·L F BissonD A Lewis
Apr 5, 1996·The Journal of Biological Chemistry·B ThorensA Porret
Sep 27, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Philipp C NettTausif Alam
Dec 19, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Oran KwonMark Levine
Feb 19, 2009·American Journal of Physiology. Endocrinology and Metabolism·Armelle LeturqueMaude Le Gall
Jul 15, 1998·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·E KintsurashviliA M Sun
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Linda S TompkinsRonald M Lynch

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.