Engineering of the hydrophobic segment of the signal sequence for efficient secretion of human lysozyme by Saccharomyces cerevisiae

Biochemical and Biophysical Research Communications
Y YamamotoM Ikehara

Abstract

To elucidate the structure-function relationship of the signal sequence for the secretion of human lysozyme by Saccharomyces cerevisiae, we have systematically engineered the hydrophobic segment using the signal sequence of chicken lysozyme. Replacement of Cys 10 with leucine caused a 1.6 times increase in the secretion of human lysozyme. An idealized signal sequence L10 in which 10 consecutive leucines were distributed from the 3rd to the 12th position was 1.8 times as effective as the native sequence. L10 can be generalized as Ln = Met-Arg-(Leu)n-Pro-Leu-Ala-Ala-Leu-Gly, where n = 10. We have also studied the secretory capability of Ln, where n = 6,8,12, and 14, and found that the length, as well as hydrophobicity, of the hydrophobic segment is an important factor in the secretion of human lysozyme by yeast.

References

Jun 15, 1987·Biochemical and Biophysical Research Communications·K YoshimuraM Ikehara
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S D Emr, T J Silhavy
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A MiyanoharaK Matsubara
Feb 1, 1980·European Journal of Biochemistry·G von Heijne

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Citations

May 1, 1990·The Journal of Membrane Biology·G von Heijne
Oct 1, 1990·Current Opinion in Biotechnology·P Tekamp-Olson, P Valenzuela
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M KikuchiM Ikehara
Dec 15, 1992·European Journal of Biochemistry·H NagahoraY Jigami
Mar 28, 2013·Critical Reviews in Biochemistry and Molecular Biology·Tslil Ast, Maya Schuldiner
Feb 7, 1989·Biochemistry·L M Gierasch
Jul 15, 1994·European Journal of Biochemistry·M F Martin-EauclaireB Svensson
Jun 13, 2019·The Protein Journal·Yuanyuan ChenRoss E Dalbey
Apr 8, 1998·The Journal of Peptide Research : Official Journal of the American Peptide Society·C DuY Z Lin
Mar 5, 1992·Journal of Molecular Biology·S L Rusch, D A Kendall
Nov 27, 2004·Journal of Molecular Recognition : JMR·Rachel Daly, Milton T W Hearn
May 16, 1988·Biochemical and Biophysical Research Communications·Y TaniyamaM Ikehara

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