Engineering Organelle-Specific Molecular Viscosimeters Using Aggregation-Induced Emission Luminogens for Live Cell Imaging

Analytical Chemistry
Wen ChenJian-Hui Jiang

Abstract

Subcellular viscosity is essential for cell functions and may indicate its physiological status. We screen two fluorescent probes by engineering tetraphenylethene (TPE) for measuring viscosity in mitochondria and lysosomes, respectively. These two probes are only weakly emissive in nonviscous medium and the emission signals are greatly enhanced in viscous medium due to the restriction of intramolecular motion. The presence of pyridium has endowed one probe with mitochondrial specificity, while the presence of indole ring has granted the other probe with lysosome-targeting ability. Their optical properties are characterized in vitro and their applications in imaging viscosity variations in mitochondria and lysosomes are also demonstrated in living cells under different stimulated processes. In addition, an increase in both mitochondrial and lysosomal viscosity during mitophagy was revealed for the first time with our probes. To our knowledge, this is the first time that TPE is engineered to be fluorescent molecular viscosimeters that possess desirable aqueous solubility, red-shifted emission, and organelle specificity.

References

Oct 25, 2006·Alimentary Pharmacology & Therapeutics·P ClavéM Serra-Prat
May 7, 2008·Journal of the American Chemical Society·Marina K KuimovaKlaus Suhling
Jul 30, 2011·Chemical Society Reviews·Yuning HongBen Zhong Tang
Feb 3, 2012·Angewandte Chemie·Hee Jung ParkBong Rae Cho
Dec 19, 2012·Journal of the American Chemical Society·Chris Wai Tung LeungBen Zhong Tang
Feb 16, 2013·Journal of the American Chemical Society·Lu WangLiezheng Deng
May 30, 2013·Journal of the American Chemical Society·Zhigang YangJong Seung Kim
Sep 30, 2014·Nature Biotechnology·Michele CastellanaNed S Wingreen
Mar 28, 2017·Nature Cell Biology·Yuchuan MiaoPeter N Devreotes
Jul 12, 2017·Chemical Society Reviews·Dongdong SuYoung-Tae Chang
Sep 17, 2017·Trends in Biotechnology·Yimin WangYixiang Duan
Jan 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xinbo SongYi Xiao

❮ Previous
Next ❯

Citations

Jan 3, 2019·Chemistry, an Asian Journal·Weijie ZhangBen Zhong Tang
Jun 19, 2019·Organic & Biomolecular Chemistry·Tong ChenShaobing Zheng
Sep 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chenggong MaBaoxing Shen
Feb 9, 2021·Organic & Biomolecular Chemistry·Tarushyam MukherjeeSriram Kanvah
Nov 7, 2020·Chemical Society Reviews·Xuechen LiWeiying Lin
May 3, 2021·Analytical and Bioanalytical Chemistry·Yuewei ZhangChuan Dong
Jun 7, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lingfeng XuLimin Liu
Jun 11, 2020·Analytical Chemistry·Peng GaoBo Tang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.