Engineering rice with lower grain arsenic

Plant Biotechnology Journal
Fenglin DengWon-Yong Song

Abstract

Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the amount of As entering the food chain. We developed transgenic rice plants expressing two different vacuolar As sequestration genes, ScYCF1 and OsABCC1, under the control of the RCc3 promoter in the root cortical and internode phloem cells, along with a bacterial γ-glutamylcysteine synthetase driven by the maize UBI promoter. The transgenic rice plants exhibited reduced root-to-shoot and internode-to-grain As translocation, resulting in a 70% reduction in As accumulation in the brown rice without jeopardizing agronomic traits. This technology could be used to reduce As intake, particularly in populations of South East Asia suffering from As toxicity and thereby improve human health.

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Citations

Aug 14, 2019·Journal of Experimental Botany·Stephan Clemens
Aug 9, 2020·Frontiers in Plant Science·Beibei HuZhong-Hua Chen
Aug 15, 2020·Frontiers in Genetics·Priyanka DasArun Lahiri Majumder
Nov 15, 2020·Journal of Hazardous Materials·Fenglin DengZhong-Hua Chen
Mar 1, 2021·Environmental Pollution·Ashish Kumar SrivastavaPenna Suprasanna
Mar 4, 2021·Nature Communications·Sheng-Kai SunFang-Jie Zhao
Aug 25, 2021·The Science of the Total Environment·Debojyoti MoulickSubhas Chandra Santra
Oct 9, 2021·Plant, Cell & Environment·Dorina Podar, Frans J M Maathuis

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Methods Mentioned

BETA
transgenic
PCR
dissecting

Software Mentioned

Excel
GraphPad Prism
ImageJ

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