Engineering Smut Resistance in Maize by Site-Directed Mutagenesis of LIPOXYGENASE 3

Frontiers in Plant Science
Krishna Mohan PathiJochen Kumlehn

Abstract

Biotic stresses caused by microbial pathogens impair crop yield and quality if not restricted by expensive and often ecologically problematic pesticides. For a sustainable agriculture of tomorrow, breeding or engineering of pathogen-resistant crop varieties is therefore a major cornerstone. Maize is one of the four most important cereal crops in the world. The biotrophic fungal pathogen Ustilago maydis causes galls on all aerial parts of the maize plant. Biotrophic pathogens like U. maydis co-evolved with their host plant and depend during their life cycle on successful manipulation of the host's cellular machinery. Therefore, removing or altering plant susceptibility genes is an effective and usually durable way to obtain resistance in plants. Transcriptional time course experiments in U. maydis-infected maize revealed numerous maize genes being upregulated upon establishment of biotrophy. Among these genes is the maize LIPOXYGENASE 3 (LOX3) previously shown to be a susceptibility factor for other fungal genera as well. Aiming to engineer durable resistance in maize against U. maydis and possibly other pathogens, we took a Cas endonuclease technology approach to generate loss of function mutations in LOX3. lox3 maize mutant pl...Continue Reading

References

Jan 1, 1995·Molecular Plant-microbe Interactions : MPMI·S Mittal, K R Davis
Aug 11, 2001·Molecular Plant-microbe Interactions : MPMI·R A WilsonN P Keller
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 12, 2002·Annual Review of Plant Biology·Ivo Feussner, Claus Wasternack
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jianping CuiFrederick M Ausubel
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Feb 6, 2007·Trends in Microbiology·Dimitrios I Tsitsigiannis, Nancy P Keller
Jul 10, 2007·The Plant Cell·Lázaro Molina, Regine Kahmann
Apr 22, 2008·Nucleic Acids Research·Andreas R GruberIvo L Hofacker
Jun 20, 2008·The Plant Journal : for Cell and Molecular Biology·Gunther DoehlemannJörg Kämper
Sep 24, 2008·The Plant Journal : for Cell and Molecular Biology·Matthew J ChristiansRichard D Vierstra
Jul 9, 2009·International Journal of Plant Genomics·Goetz HenselJochen Kumlehn
Jul 1, 2006·Molecular Plant Pathology·Marion Brodhagen, Nancy P Keller
Jun 30, 2010·Nature Reviews. Genetics·Peter N Dodds, John P Rathjen
Oct 7, 2011·Nature·Armin DjameiRegine Kahmann
Mar 29, 2012·The Plant Cell·Karina van der LindeGunther Doehlemann
Mar 31, 2012·Journal of Plant Physiology·Alessandro ManoliAlberto Nonis
May 17, 2012·PLoS Pathogens·Christoph HemetsbergerGunther Doehlemann
Jan 22, 2013·Plant Signaling & Behavior·Magdalena HilbertAlga Zuccaro
Jan 26, 2013·The Plant Journal : for Cell and Molecular Biology·Michael RiemannMoritoshi Iino
Aug 21, 2013·Science·Jeffery L DanglBrian J Staskawicz
Oct 12, 2013·The Plant Journal : for Cell and Molecular Biology·Holger Puchta, Friedrich Fauser
Jun 13, 2014·The Plant Cell·Kemal Kazan, Rebecca Lyons
Jul 6, 2014·PLoS Pathogens·Thomas BrefortRegine Kahmann
Sep 4, 2014·Nature Biotechnology·John G DoenchDavid E Root
Jun 16, 2015·Molecular Plant-microbe Interactions : MPMI·Vamsi J NalamJyoti Shah
Jun 26, 2015·Journal of Experimental Botany·Muhammad NaseemThomas Dandekar

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Citations

Apr 10, 2021·Journal of Zhejiang University. Science. B·Chao LiBaohong Zhang
Aug 21, 2021·International Journal of Biological Macromolecules·Yu KangXin He

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Methods Mentioned

BETA
Knockout
phosphotransferase
transgenic
PCR
confocal microscopy
biopsy
and
Confocal

Software Mentioned

A plasmid Editor ( ApE )
- macro
DESKGEN
qpcrmix
RNAfold
Clone Manager
ImageJ
R

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