Engineering synergetic CO2 -fixing pathways for malate production

Metabolic Engineering
Guipeng HuLiming Liu

Abstract

Increasing the microbial CO2-fixing efficiency often requires supplying sufficient ATP and redirecting carbon flux for the production of metabolites. However, addressing these two issues concurrently remains a challenge. Here, we present a combinational strategy based on a synergetic CO2-fixing pathway that combines an ATP-generating carboxylation reaction in the central metabolic pathway with the ATP-consuming RuBisCO shunt in the carbon fixation pathway. This strategy provides enough ATP to improve the efficiency of CO2 fixation and simultaneously rewires the CO2-fixing pathway to the central metabolic pathway for the biosynthesis of chemicals. We demonstrate the application of this strategy by increasing the CO2-fixing rate and malate production in the autotroph Synechococcus elongatus by 110% and to 260 μM respectively, as well as increasing these two factors in the heterotrophic CO2-fixing Escherichia coli by 870% and to 387 mM respectively.

Citations

Aug 23, 2019·Nature Communications·Cong GaoLiming Liu
May 10, 2020·Nature Communications·Qiang DingXiulai Chen
Feb 6, 2021·Critical Reviews in Biotechnology·Hui LiuLiming Liu
Feb 15, 2021·Biotechnology Advances·Wenna LiQipeng Yuan
Apr 6, 2021·Applied Microbiology and Biotechnology·Lihao JiLiming Liu
Jul 19, 2021·Microbiological Research·Ayesha NisarSardar Azhar Mehmood

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