Engineering synthetic bacterial consortia for enhanced desulfurization and revalorization of oil sulfur compounds

Metabolic Engineering
Igor MartínezEduardo Díaz

Abstract

The 4S pathway is the most studied bioprocess for the removal of the recalcitrant sulfur of aromatic heterocycles present in fuels. It consists of three sequential functional units, encoded by the dszABCD genes, through which the model compound dibenzothiophene (DBT) is transformed into the sulfur-free 2-hydroxybiphenyl (2HBP) molecule. In this work, a set of synthetic dsz cassettes were implanted in Pseudomonas putida KT2440, a model bacterial "chassis" for metabolic engineering studies. The complete dszB1A1C1-D1 cassette behaved as an attractive alternative - to the previously constructed recombinant dsz cassettes - for the conversion of DBT into 2HBP. Refactoring the 4S pathway by the use of synthetic dsz modules encoding individual 4S pathway reactions revealed unanticipated traits, e.g., the 4S intermediate 2HBP-sulfinate (HBPS) behaves as an inhibitor of the Dsz monooxygenases, and once secreted from the cells it cannot be further taken up. That issue should be addressed for the rational design of more efficient biocatalysts for DBT bioconversions. In this sense, the construction of synthetic bacterial consortia to compartmentalize the 4S pathway into different cell factories for individual optimization was shown to enhan...Continue Reading

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Citations

Jun 1, 2016·Metabolic Engineering·Haoran Zhang, Xiaonan Wang
Apr 28, 2017·Environmental Microbiology·Matteo CavaliereJosé I Jiménez
Sep 12, 2018·Applied Microbiology and Biotechnology·Shuiquan ChenYunbo Zhang
Apr 19, 2020·Journal of Industrial Microbiology & Biotechnology·Lu LiShuli Liang
Oct 14, 2020·Environmental Science and Pollution Research International·Hussein N NassarNour Sh El-Gendy
Oct 22, 2020·Nature Communications·Eveline-Marie LammensRob Lavigne
Dec 29, 2020·Microbial Biotechnology·Lisa BretschneiderRohan Karande
Dec 22, 2019·Biotechnology Advances·Xiujuan QianKatrin Ochsenreither
Mar 22, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marinos StylianouAgapios Agapiou
Jun 8, 2021·Computational and Structural Biotechnology Journal·Carlos Molina-SantiagoDiego Romero
Jul 5, 2021·Journal of Hazardous Materials·Kha Mong TranDokyun Na
Jun 29, 2021·Critical Reviews in Biochemistry and Molecular Biology·Matthew WongMattheos Koffas

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