Engineering the Autotroph Methanococcus maripaludis for Geraniol Production

ACS Synthetic Biology
Zhe LyuWilliam B Whitman

Abstract

The rapid autotrophic growth of the methanogenic archaeon Methanococcus maripaludis on H2 and CO2 makes it an attractive microbial chassis to inexpensively produce biochemicals. To explore this potential, a synthetic gene encoding geraniol synthase (GES) derived from Ocimum basilicum was cloned into a M. maripaludis expression vector under selection for puromycin resistance. Recombinant expression of GES in M. maripaludis during autotrophic growth on H2/CO2 or formate yielded geraniol at 2.8 and 4.0 mg g(-1) of dry weight, respectively. The yield of geraniol decreased 2-3-fold when organic carbon sources were added to stimulate heterotrophic growth. In the absence of puromycin, geraniol production during autotrophic growth on formate increased to 4.6 mg g(-1) of dry weight. A conceptual model centered on the autotrophic acetyl coenzyme A biosynthetic pathway identified strategies to divert more autotrophic carbon flux to geraniol production.

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Citations

Jun 5, 2016·Cellular and Molecular Life Sciences : CMLS·Zhe Lyu, William B Whitman
Jul 28, 2016·Current Opinion in Chemical Biology·Oren YishaiArren Bar-Even
Nov 2, 2016·Biotechnology and Bioengineering·Michael J McAnultyThomas K Wood
Aug 23, 2017·BMC Genomics·Sean P GilmoreMichelle A O'Malley
Jan 18, 2018·Journal of Bacteriology·Zhe LyuWilliam B Whitman
Jan 18, 2018·Extremophiles : Life Under Extreme Conditions·Tsubasa FukeHaruyuki Atomi
Jan 24, 2019·Extremophiles : Life Under Extreme Conditions·Geraldy L S LimanThomas J Santangelo
Jan 6, 2018·AMB Express·Franziska EnzmannDirk Holtmann
Jun 12, 2016·Microbial Cell Factories·Nishu GoyalIftekhar A Karimi
Sep 25, 2019·Microbial Cell Factories·Chuanbo ZhangWenyu Lu
Jul 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wanda MączkaMałgorzata Grabarczyk
Nov 10, 2018·Biotechnology for Biofuels·Annalisa Abdel AzimGünther Bochmann
Mar 1, 2020·Extremophiles : Life Under Extreme Conditions·Takaaki SatoHaruyuki Atomi
Dec 4, 2020·Biotechnology Advances·Kevin PfeiferSimon K-M R Rittmann
Jan 17, 2021·Applied and Environmental Microbiology·Jared AldridgeNicole R Buan
Mar 16, 2019·Current Opinion in Biotechnology·Zhe Lyu, William B Whitman

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