PMID: 11607155Mar 1, 1991Paper

Engineering the chloroplast genome: techniques and capabilities for chloroplast transformation in Chlamydomonas reinhardtii

Proceedings of the National Academy of Sciences of the United States of America
K L KindleD B Stern

Abstract

Chloroplast transformation of Chlamydomonas reinhardtii has been accomplished by agitating cell wall-deficient cells in the presence of glass beads and DNA. By using the atpB gene as the selected marker and cells grown in 0.5 mM 5-fluorodeoxyuridine, we have recovered up to 50 transformants per microgram of DNA. This method is easy and does not require specialized equipment, although it is not as efficient as the tungsten particle bombardment method [Boynton, J. E., Gillham, N. W., Harris, E. H., Hosler, J. P., Johnson, A. M., Jones, A. R., Randolph-Anderson, B. L., Robertson, D., Klein, T. M., Shark, K. B. & Sanford, J. C. (1988) Science 240, 1534-1537]. By using particle bombardment, we have developed a cotransformation approach in which spectinomycin-resistant 16S rRNA-encoding DNA is the selected marker, and we have demonstrated that cotransformation of an unselected marker on an independent replicon is very efficient. We have used this strategy (i) to recover transformants with partially deleted atpB genes that could not otherwise have been selected since they did not restore photosynthetic capability to a recipient carrying a more extensive atpB deletion and (ii) to generate specific deletion mutations in a wild-type reci...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H S ShepherdN W Gillham
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K L Kindle
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S P Mayfield, K L Kindle
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D R DienerJ L Rosenbaum
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Z SvabP Maliga
Jan 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·N Sueoka

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Citations

Nov 1, 1991·Molecular & General Genetics : MGG·S M NewmanJ E Boynton
Jun 12, 1996·Molecular & General Genetics : MGG·N FischerJ D Rochaix
Dec 22, 2010·Sexual Plant Reproduction·Armin Hallmann
Oct 1, 1993·Current Opinion in Biotechnology·T M Klein, S Fitzpatrick-McElligott
Aug 27, 1998·Molecular and Biochemical Parasitology·S SbicegoB Blum
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H CeruttiA T Jagendorf
Sep 21, 2001·Journal of Molecular Biology·R Bock
Oct 1, 1992·Current Opinion in Genetics & Development·J D Rochaix
Sep 25, 2015·The Plant Journal : for Cell and Molecular Biology·Rouhollah BarahimipourRalph Bock
Jul 28, 2009·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Huan-Huan WangZan-Min Hu
Mar 7, 2007·Current Opinion in Biotechnology·Kerry A Lutz, Pal Maliga
Mar 24, 2011·Plant Biotechnology Journal·Anil Day, Michel Goldschmidt-Clermont
Jun 8, 2012·Biotechnology and Bioengineering·Samaneh Noor-MohammadiTyler W Johannes
Oct 24, 2013·The Plant Journal : for Cell and Molecular Biology·Lars B Scharff, Ralph Bock
Sep 25, 2015·Journal of Experimental Botany·Doris GanglColin Robinson
Feb 3, 2015·The Plant Journal : for Cell and Molecular Biology·Mark A ScaifeAlison G Smith
Jan 1, 2012·Computational and Structural Biotechnology Journal·Azadeh Pourmir, Tyler W Johannes
May 6, 2016·Frontiers in Plant Science·Lior DoronMichal Shapira
Apr 1, 2017·Biotechnology & Genetic Engineering Reviews·Shabnam Shamriz, Hamideh Ofoghi
Mar 2, 2017·Cold Spring Harbor Perspectives in Biology·Christian R BoehmJim Haseloff
Aug 10, 2004·Applied Microbiology and Biotechnology·Otto Pulz, Wolfgang Gross
May 24, 2017·Molecular Biology and Evolution·Ilkka KronholmSinéad Collins

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