Engineering the ovarian cycle using in vitro follicle culture

Human Reproduction
Robin M SkoryTeresa K Woodruff

Abstract

Can cultured follicles model the ovarian cycle, including follicular- and luteal-phase hormone synthesis patterns and ovulation? Under gonadotrophin stimulation, murine follicles grown in an encapsulated three-dimensional system ovulate in vitro and murine and human follicle hormone synthesis mimics follicular and luteal phases expected in vivo. Studies of the human ovary and follicle function are limited by the availability of human tissue and lack of in vitro models. We developed an encapsulated in vitro follicle growth (eIVFG) culture system, which preserves 3D follicular structure. Thus far, the alginate system has supported the culture of follicles from mice, dog, rhesus macaque, baboon and human. These studies have shown that cultured follicles synthesize steroid hormones similar to those observed during the follicular phase in vivo. Cultured murine follicles were treated with human chorionic gonadotrophin (hCG) and epidermal growth factor (EGF) and either assayed for luteinization or removed from alginate beads and assayed for ovulation. Human follicles were also cultured, treated with follicle-stimulating hormone (FSH), hCG and EGF to mimic gonadotrophin changes throughout the ovarian cycle, and culture medium was assay...Continue Reading

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Citations

Sep 9, 2015·Human Reproduction·Kazuhiro KawamuraAaron J Hsueh
Jul 2, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Teresa K Woodruff
Aug 5, 2016·Journal of Ovarian Research·Matthew R ZanotelliSana M Salih
Nov 3, 2016·Journal of Tissue Engineering and Regenerative Medicine·Yong Jin KimSeung-Yup Ku
Dec 29, 2016·Annals of Biomedical Engineering·Anu DavidAriella Shikanov
Mar 30, 2017·Nature Communications·Shuo XiaoTeresa K Woodruff
Jun 9, 2017·Zygote : the Biology of Gametes and Early Embryos·Maxim FilatovMaria Semenova
Jul 17, 2018·Endocrinology·Rebecca L RobkerDarryl L Russell
Feb 5, 2019·Acta Obstetricia Et Gynecologica Scandinavica·Mahmoud Salama, Teresa K Woodruff
Nov 12, 2019·Reproduction, Fertility, and Development·Xiaolei YaoPeihua You
Apr 7, 2020·Nature Biomedical Engineering·Emma S GargusTeresa K Woodruff
May 15, 2020·Biology of Reproduction·Leah E SimonFrancesca E Duncan
Mar 29, 2017·Human Reproduction Open·Richard A AndersonEvelyn E Telfer
Mar 2, 2017·Annals of Biomedical Engineering·Julie Vanacker, Christiani A Amorim
Sep 22, 2017·Journal of Assisted Reproduction and Genetics·Mili ThakurElizabeth E Puscheck
Mar 11, 2016·Reproductive Medicine and Biology·Lalitha Devi, Sandeep Goel
May 10, 2019·Clinical Medicine Insights. Reproductive Health·Michael von WolffFrank Nawroth
Sep 29, 2019·International Journal of Molecular Sciences·Alexander A TokmakovKen-Ichi Sato
Oct 28, 2019·International Journal of Molecular Sciences·Zaza Abidin, Eileen P Treacy
Apr 11, 2020·Reproductive Medicine and Biology·Ken-Ichi Sato, Alexander A Tokmakov
Mar 23, 2017·Annals of Biomedical Engineering·Xiaoming He
Jul 12, 2019·Biology of Reproduction·Katsuhiko Hayashi
Oct 7, 2017·Advanced Healthcare Materials·Janna NawrothPeter Loskill
Nov 26, 2020·Tissue Engineering and Regenerative Medicine·Russel C SequeiraJames J Yoo
Dec 15, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Katie L LandPatrick R Hannon
Dec 29, 2020·Frontiers in Cell and Developmental Biology·Alexander A TokmakovKen-Ichi Sato
Apr 8, 2021·Fertility and Sterility·Evelyn E Telfer, Claus Yding Andersen
Jun 8, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Du XiangYanfeng Wang
Apr 15, 2021·Endocrinology·Vedant V Bodke, Joanna E Burdette

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