Engineering the unicellular alga Phaeodactylum tricornutum for high-value plant triterpenoid production

Plant Biotechnology Journal
Sarah D'AdamoMichiel Matthijs

Abstract

Plant triterpenoids constitute a diverse class of organic compounds that play a major role in development, plant defence and environmental interaction. Several triterpenes have demonstrated potential as pharmaceuticals. One example is betulin, which has shown promise as a pharmaceutical precursor for the treatment of certain cancers and HIV. Major challenges for triterpenoid commercialization include their low production levels and their cost-effective purification from the complex mixtures present in their natural hosts. Therefore, attempts to produce these compounds in industrially relevant microbial systems such as bacteria and yeasts have attracted great interest. Here, we report the production of the triterpenes betulin and its precursor lupeol in the photosynthetic diatom Phaeodactylum tricornutum, a unicellular eukaryotic alga. This was achieved by introducing three plant enzymes in the microalga: a Lotus japonicus oxidosqualene cyclase and a Medicago truncatula cytochrome P450 along with its native reductase. The introduction of the L. japonicus oxidosqualene cyclase perturbed the mRNA expression levels of the native mevalonate and sterol biosynthesis pathway. The best performing strains were selected and grown in a 550...Continue Reading

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Citations

May 10, 2019·Marine Drugs·Chiara LauritanoAlessandra Rogato
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Oct 19, 2021·Frontiers in Bioengineering and Biotechnology·Gilles DefrelFayza Daboussi

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Datasets Mentioned

BETA
AF219942
NP178018.1
AB181245
DQ335781
X73149

Methods Mentioned

BETA
glycosylations
genetic modification
transgenic
PCRs
PCR

Software Mentioned

R
CFX Manager
agricolae

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