Engineering transkingdom signalling in plants to control gene expression in rhizosphere bacteria

Nature Communications
Barney A GeddesPhilip S Poole

Abstract

The root microbiota is critical for agricultural yield, with growth-promoting bacteria able to solubilise phosphate, produce plant growth hormones, antagonise pathogens and fix N2. Plants control the microorganisms in their immediate environment and this is at least in part through direct selection, the immune system, and interactions with other microorganisms. Considering the importance of the root microbiota for crop yields it is attractive to artificially regulate this environment to optimise agricultural productivity. Towards this aim we express a synthetic pathway for the production of the rhizopine scyllo-inosamine in plants. We demonstrate the production of this bacterial derived signal in both Medicago truncatula and barley and show its perception by rhizosphere bacteria, containing bioluminescent and fluorescent biosensors. This study lays the groundwork for synthetic signalling networks between plants and bacteria, allowing the targeted regulation of bacterial gene expression in the rhizosphere for delivery of useful functions to plants.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Sep 1, 1974·Journal of General Microbiology·J E Beringer
May 24, 2005·Nature Methods·Kyoung-Hee ChoiHerbert P Schweizer
Nov 1, 1996·Applied and Environmental Microbiology·D M GordonP J Murphy
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P J MurphyJ Schell
Jul 6, 2006·Applied and Environmental Microbiology·Robert M Q ShanksGeorge A O'Toole
Apr 5, 2007·Nature Protocols·Kyoung-Hee Choi, Herbert P Schweizer
Apr 5, 2007·Nature Protocols·Jan LisecAlisdair R Fernie
Feb 9, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·K E Van StraatenD A R Sanders
Nov 6, 2008·PloS One·Carola EnglerSylvestre Marillonnet
Nov 7, 2008·Methods in Molecular Biology·Nick S BerrowRaymond J Owens
May 30, 2009·Plasmid·Robert M Q ShanksGeorge A O'Toole
Jul 7, 2009·Annual Review of Microbiology·Ben Lugtenberg, Faina Kamilova
Mar 3, 2011·PloS One·Ernst WeberSylvestre Marillonnet
May 10, 2011·Science·Rodrigo MendesJos M Raaijmakers
Aug 4, 2012·Nature·Derek S LundbergJeffery L Dangl
Nov 19, 2013·Methods in Molecular Biology·Wendy A Harwood
Jan 28, 2015·Current Opinion in Biotechnology·Barney A GeddesPhilip Poole
Apr 25, 2015·Journal of Experimental Botany·Andrzej Tkacz, Philip Poole
Dec 4, 2015·Nature·Yang BaiPaul Schulze-Lefert
Dec 8, 2015·Nature Chemical Biology·Clement T Y ChanJames J Collins
Mar 16, 2017·Nature·Gabriel CastrilloJeffery L Dangl
May 13, 2017·Plant Physiology·Francesco PiniPhilip S Poole
Oct 4, 2017·Nature Plants·Annalisa RizzaAlexander M Jones

❮ Previous
Next ❯

Citations

Aug 15, 2020·Environmental Microbiology·María Victoria Berlanga-ClaveroDiego Romero
Jan 29, 2020·Nature Reviews. Microbiology·Rekha Seshadri
Aug 17, 2020·Plants·Abdoulaye SoumareLamfeddal Kouisni
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sylwia ZielińskaAdam Matkowski
Dec 18, 2019·Nature Microbiology·Min-Hyung RyuChristopher A Voigt
Jan 17, 2020·Plants·Kishan MahmudAli Missaoui
Nov 25, 2020·The ISME Journal·Timothy L HaskettPhilip S Poole
Jan 26, 2021·Current Opinion in Biotechnology·Joyati DasGopaljee Jha
Jan 19, 2021·Microbiological Research·Janie ZhangZhenyu Cheng
Mar 10, 2021·Nature Plants·Elizabeth FrenchLaramy Enders
Mar 8, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Seema SheoranSujay Rakshit
Nov 18, 2020·Current Opinion in Biotechnology·Kent M RappMichael J Betenbaugh
Mar 4, 2021·The New Phytologist·Pankaj TrivediJan E Leach
Jan 23, 2021·Annual Review of Plant Biology·Yameng LiangFeng Tian
Jun 2, 2021·Current Opinion in Microbiology·Giansimone PerrinoGuy-Bart Stan
Aug 16, 2021·Current Opinion in Biotechnology·Matteo ChialvaPaola Bonfante
Aug 18, 2021·Trends in Plant Science·Rui-Peng YuLong Li
Oct 1, 2021·ACS Synthetic Biology·Tim Schnabel, Elizabeth Sattely
Nov 13, 2021·Microbial Biotechnology·Hang-Wei HuJi-Zheng He
Dec 2, 2021·Natural Product Reports·Jack A ConnollyRainer Breitling

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensor
transgenic
nuclear
X-ray
fluorescence
PCR
restriction digest
electrophoresis
confocal microscopy

Software Mentioned

IMARIS
R
Adobe Illustrator
BLAST
programming
ImageJ
IndiGO
IMG Ortholog Neighbourhood Viewer
MassHunter
IMARIS Xtension

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Zentralblatt Für Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Zweite Naturwissenschaftliche Abt.: Allgemeine, Landwirtschaftliche Und Technische Mikrobiologie
P Apel, G Weichsel
Zentralblatt Für Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Zweite Naturwissenschaftliche Abt.: Allgemeine, Landwirtschaftliche Und Technische Mikrobiologie
P Apel, G Weichsel
Frontiers in Plant Science
Senga Robertson-AlbertynDavide Bulgarelli
© 2021 Meta ULC. All rights reserved