Engineering Tunable Dual Functional Protein Cage Nanoparticles Using Bacterial Superglue

Biomacromolecules
Yoonji BaeSebyung Kang

Abstract

The selective detection of specific cells of interest and their effective visualization is important but challenging, and fluorescent cell imaging with target-specific probes is commonly used to visualize cell morphology and components and to track cellular processes. Multiple displays of two or more targeting ligands on a polyvalent single template would make it possible to construct versatile multiplex fluorescent cell imaging probes that can visualize two or more target cells individually without the need for a set of individual probes. To achieve this goal, we used encapsulin, a new class of protein cage nanoparticles, as a template and implanted dual targeting capability by presenting two different affibody molecules on a single encapsulin protein cage nanoparticle post-translationally. Encapsulin was self-assembled from 60 identical subunits to form a hollow and symmetric spherical structure with a uniform size. We genetically inserted SpyTag peptides onto the encapsulin surface and prepared various SpyCatcher-fused proteins, such as fluorescent proteins and targeting affibody molecules. We successfully displayed fluorescent proteins and affibody molecules together on a single encapsulin in a mix-and-match manner post-tra...Continue Reading

References

Jan 28, 2009·Nature Structural & Molecular Biology·Markus SutterNenad Ban
Aug 29, 2009·Angewandte Chemie·Ellen M Sletten, Carolyn R Bertozzi
Jan 7, 2010·Chembiochem : a European Journal of Chemical Biology·Ilya LyakhovJacek Capala
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Emilia S OlsonRoger Y Tsien
May 11, 2010·Chembiochem : a European Journal of Chemical Biology·Deboshri BanerjeeM G Finn
Dec 15, 2010·Biomaterials·Jinhao GaoZhen Cheng
Apr 9, 2011·Journal of the American Chemical Society·Toru KomatsuYasuteru Urano
Jan 31, 2012·Advanced Drug Delivery Reviews·Yujie MaJeroen J L M Cornelissen
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bijan ZakeriMark Howarth
Nov 23, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Jae-A HanSebyung Kang
Feb 18, 2014·Journal of the American Chemical Society·W Frederik RurupJeroen J L M Cornelissen
May 13, 2014·Angewandte Chemie·Christopher SchoeneMark Howarth
Jun 5, 2015·Current Opinion in Chemical Biology·Jhon Ralph EnterinaRobert E Campbell
Feb 9, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Rindia M PutriNathalie Katsonis
Apr 28, 2016·Journal of Nanobiotechnology·Susan ThraneAdam F Sander
Nov 18, 2016·Chemical Communications : Chem Comm·Hyojin MoonSebyung Kang
May 18, 2017·Chembiochem : a European Journal of Chemical Biology·Veronica PessinoBo Huang
Sep 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hyojin MoonSebyung Kang

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Citations

Mar 16, 2019·Scientific Reports·Anne-Marie C AnderssonMark Howarth
Jul 23, 2019·Biotechnology and Bioengineering·Hansol KimSebyung Kang
Sep 13, 2020·Biotechnology and Bioengineering·Jesse A Jones, Tobias W Giessen
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Jin Xiang WongBernd H A Rehm
Feb 2, 2019·International Journal of Molecular Sciences·Huimei RenGuojun Zheng
May 1, 2020·Biomaterials Science·Nicole F SteinmetzFrank Sainsbury
Oct 13, 2020·Frontiers in Bioengineering and Biotechnology·Shin-Lei PengYu-Hsin Lin
Nov 22, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Alessandro GroazAllen P Liu
Feb 8, 2021·International Journal of Biological Macromolecules·Yoonji BaeSebyung Kang
May 28, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hansol KimSebyung Kang
Jul 3, 2021·Nanomaterials·Javier M RodríguezJosé R Castón
Sep 18, 2021·Angewandte Chemie·Jesse A JonesTobias W Giessen
Nov 9, 2021·Biomacromolecules·Sandra Michel-SouzyJeroen J L M Cornelissen

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