Engineering virus resistance via CRISPR-Cas systems

Current Opinion in Virology
Ahmed Mahas, Magdy Mahfouz

Abstract

In prokaryotes, CRISPR/Cas adaptive immunity systems target and destroy nucleic acids derived from invading bacteriophages and other foreign genetic elements. In eukaryotes, the native function of these systems has been exploited to combat viruses in mammals and plants. Rewired CRISPR/Cas9 and CRISPR/Cas13 systems have been used to confer resistance against DNA and RNA viruses, respectively. Here, we discuss recent approaches employing CRISPR/Cas systems to combat viruses in eukaryotes, highlight key challenges, and provide future perspectives. Moreover, we discuss the application of CRISPR/Cas systems in genome-wide screens to identify key host factors for virus infection to enhance our understanding of basic virus biology and to identify and study virus-host interactions.

Citations

Feb 26, 2019·Plant Biotechnology Journal·Xiaohui ZhanJiang Zhang
Oct 24, 2019·Journal of Experimental Botany·Syed Adeel ZafarAshwani Pareek
Mar 3, 2019·Antibiotics·Alexandre Loureiro, Gabriela Jorge da Silva
Mar 12, 2019·Frontiers in Microbiology·Shweta JaiswalPratyoosh Shukla
Jul 28, 2019·The Plant Journal : for Cell and Molecular Biology·Alberto CarbonellJosé-Antonio Daròs
Dec 4, 2019·Genome Biology·Ahmed MahasMagdy Mahfouz
Oct 17, 2019·Plant Biotechnology Journal·Yaling ZhaoTong Zhang
Jan 23, 2021·Viruses·Carla Mr VarandaPatrick Materatski
Nov 17, 2020·Frontiers in Microbiology·Yongsen CaoFangfang Li
May 11, 2021·Molecular Therapy. Methods & Clinical Development·Ekapot SingsuksawatBunpote Siridechadilok
Jun 3, 2021·International Journal of Molecular Sciences·Antonia MoresDaniela Marone
Apr 24, 2021·Annual Review of Phytopathology·Xiuling YangAiming Wang

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