Global transcription machinery engineering (gTME) is an approach for reprogramming gene transcription to elicit cellular phenotypes important for technological applications. Here we show the application of gTME to Saccharomyces cerevisiae for improved glucose/ethanol tolerance, a key trait for many biofuels programs. Mutagenesis of the transcription factor Spt15p and selection led to dominant mutations that conferred increased tolerance and more efficient glucose conversion to ethanol. The desired phenotype results from the combined effect of three separate mutations in the SPT15 gene [serine substituted for phenylalanine (Phe(177)Ser) and, similarly, Tyr(195)His, and Lys(218)Arg]. Thus, gTME can provide a route to complex phenotypes that are not readily accessible by traditional methods.
Characterization of a negative regulator AveI for avermectin biosynthesis in Streptomyces avermitilis NRRL8165
Comparative proteome analysis of robust Saccharomyces cerevisiae insights into industrial continuous and batch fermentation
High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast?
Global transcription engineering of brewer's yeast enhances the fermentation performance under high-gravity conditions
Weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of 'Biofuel'
High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal
Identification of novel genes responsible for ethanol and/or thermotolerance by transposon mutagenesis in Saccharomyces cerevisiae
Regulatory and metabolic network of rhamnolipid biosynthesis: traditional and advanced engineering towards biotechnological production
Improving ethanol fermentation performance of Saccharomyces cerevisiae in very high-gravity fermentation through chemical mutagenesis and meiotic recombination
Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance
Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray
Enhanced thermotolerance and ethanol tolerance in Saccharomyces cerevisiae mutated by high-energy pulse electron beam and protoplast fusion
A semi-quantitative high-throughput screening method for microbial L-tyrosine production in microtiter plates
Overcoming inhibitors in a hemicellulosic hydrolysate: improving fermentability by feedstock detoxification and adaptation of Pichia stipitis
Engineering of Rhodococcus cell catalysts for tolerance improvement by sigma factor mutation and active plasmid partition
Current status, strategies, and potential for the metabolic engineering of heterologous polyketides in Escherichia coli
The yin and yang of yeast: biodiversity research and systems biology as complementary forces driving innovation in biotechnology
Development of industrial brewing yeast with low acetaldehyde production and improved flavor stability
Reverse biological engineering of hrdB to enhance the production of avermectins in an industrial strain of Streptomyces avermitilis
Rational, combinatorial, and genomic approaches for engineering L-tyrosine production in Escherichia coli
Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production
Determining the effects of inositol supplementation and the opi1 mutation on ethanol tolerance of Saccharomyces cerevisiae
Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.