Abstract
Injury to the adult optic nerve, caused mechanically or by diseases, is still not reparable because the retinal ganglion cells (RGCs) are not allowed to regrow their axons and die retrogradely, although they possess the intrinsic propensity to regenerate axons in experimental conditions. In vitro propagated embryonic stem cells derived from the early chicken neural tube (NTSCs) were used to examine whether transplanted NTSCs produce growth-promoting factors and pave the microenvironment, thus facilitating axonal regeneration within the rat optic nerve. NTSCs survived within the site where the optic nerve had been cut and continued to be nestin-positive, thus preserving their undifferentiated cell phenotype. Transplanted NTSCs activated the matrix metalloproteases (MMP)-2 and -14 in glial fibrillary acidic protein (GFAP)-positive optic nerve astrocytes. MMP2 production correlated with immunohistochemically visible degradation of inhibitory chondroitin sulfate proteoglycans (CSPGs). In addition, NTSCs produced a panoply of neurite-promoting factors including oncomodulin, ciliary neurotrophic factor, brain-derived neurotrophic factor and crystallins beta and gamma. Cut axons intermingled with NTSCs and passed through the zone of i...Continue Reading
Citations
Dec 23, 2008·Eye·T V JohnsonK R Martin
May 30, 2009·Nature Protocols·Patrick OellersSolon Thanos
Nov 21, 2012·Regenerative Medicine·Huasong GaoJianhong Zhu
Apr 10, 2013·Brain Structure & Function·Mael LeverCarsten Theiss
Jul 8, 2014·Progress in Retinal and Eye Research·Solon ThanosArndt Heiligenhaus
Mar 1, 2016·Molecular Neurobiology·Lien AndriesLies De Groef
Oct 31, 2009·Neuroscience·B CsillikE Knyihar-Csillik
Mar 26, 2009·Experimental Neurology·Isabel ZwartLing-Sun Jen
Oct 22, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Dana M García, Joseph R Koke
Nov 6, 2013·Journal of Neurochemistry·Xiaodong LiRoseline Godbout
Dec 5, 2009·The American Journal of Pathology·Takahisa KogaBeatrice Y J T Yue
Jan 27, 2015·Cytotherapy·Esra EmreErdal Karaöz
Aug 23, 2012·Neurobiology of Disease·Nina FainsteinTamir Ben-Hur
Nov 4, 2010·Biochimica Et Biophysica Acta·Valerie C FleischW Ted Allison
Aug 18, 2016·Progress in Neurobiology·Maria Egle De Stefano, Maria Trinidad Herrero
Oct 10, 2014·Cell Transplantation·Michael R R BöhmSolon Thanos
Aug 20, 2010·Cell Transplantation·Camila Zaverucha-do-ValleRosalia Mendez-Otero
Mar 18, 2016·International Journal of Molecular Medicine·Sokjoong ChungHelen Lew
Apr 30, 2019·Frontiers in Psychiatry·Xiao-Hong LiJia-Xu Chen
Jan 16, 2021·Experimental & Molecular Medicine·Jinyoung Lee, Yongcheol Cho