Engraftment of immune-deficient mice with human hematopoietic stem cells

Science
S Kamel-Reid, J E Dick

Abstract

A system in which immune-deficient mice are repopulated with cells from the human myeloid lineage, and that provides an in vivo stem cell assay for human hematopoietic cells is described. Generation of the chimeric human/immune-deficient (HID) mice was dependent on the use of immune-deficient bg/nu/xid mice. Infusion of these mice with human bone marrow gave rise to increases in human macrophage progenitors during more than 5 weeks of in vivo growth, indicating the seeding, proliferation, and differentiation of human stem cells. The human identity of the progenitors was confirmed by sequence analysis and their dependence on human growth factors. The creation of HID mice lays the foundation for establishing animal models for a wide variety of human hemopathies, from leukemia to infectious disease.

References

Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V KindlerP Vassalli
Aug 1, 1968·The American Journal of Physiology·P A ChervenickM M Wintrobe
Dec 1, 1968·Journal of Cellular Physiology·G MatioliH Niewisch

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Citations

Jan 1, 1993·Journal of Clinical Laboratory Analysis·R I FoxH I Kang
Jan 1, 1990·International Journal of Cell Cloning·H E BroxmeyerE A Boyse
Sep 1, 1992·Journal of Clinical Immunology·T S Barry, B F Haynes
Apr 1, 1995·Journal of Biomedical Science·F.Y.T. TungJ.B. Hendricks
Jan 29, 2008·International Journal of Hematology·Yoshihiro Takihara
Mar 21, 1991·Journal of Immunological Methods·S Froidevaux, F Loor
Jan 1, 1990·Life Sciences·N EndeN M Ponzio
Feb 1, 1991·Veterinary Immunology and Immunopathology·G FritschS J Black
Aug 1, 1992·Antiviral Research·J A Koch, R M Ruprecht
Oct 1, 1996·Trends in Biotechnology·T A McAdamsE T Papoutsakis
Jan 22, 2004·Archives of Medical Research·Héctor MayaniPatricia Flores-Guzmán
Feb 28, 2002·Journal of Hepatology·Chandan Guha, Jayanta Roy Chowdhury
Mar 20, 2003·Experimental Hematology·Loretta GammaitoniWanda Piacibello
Oct 6, 1999·Current Opinion in Immunology·M A Dao, J A Nolta
Aug 1, 1997·Molecular Medicine Today·R MazeD A Williams
Feb 24, 2009·Bone Marrow Transplantation·T LinW Matsui
Mar 20, 2012·Cellular & Molecular Immunology·Adam C DrakeJianzhu Chen
Aug 1, 1997·Nature Biotechnology·A M Krensky
Feb 22, 2002·Parasite Immunology·Matthew D Taylor, Kathryn J Else
Nov 25, 1998·British Journal of Haematology·J P GoldmanA J Thrasher
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W J MurphyD L Longo
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Z RatajczakA M Gewirtz
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A ChambersN Hozumi
Apr 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·D M NevilleK Srinivasachar

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