PMID: 15367358Sep 16, 2004Paper

Enhance effect of Ox-LDL on NF-kappaB activity in glomerular mesangial cells from the Zucker rats

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Wei-hua GanRong-hua Chen

Abstract

To study the effect of oxidized low-density lipoprotein (Ox-LDL) on nuclear NF-kappaB activity in cultured glomerular mesangial cells (GMCs) from the Zucker rats and the correlation between the change of NF-kappaB activity and ras's age and genotype. Four groups of GMCs (O(3m),O(10m), L(3m) and L(10m)) from the 3- and 10- month (fa/fa) and 3- and 10- month lean (Fa/Fa) Zucker rats were stimulated with Ox-LDL. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-kappaB. Gel supershift assay(GSA) was used to detect the subunit of NF-kappaB dimer. (1) NF-kappaB activity after induction with Ox-LDL in the 3 groups (O(3m),O(10m) and L(3m)) of GMCs were significantly higher than that in the control group (P<0.01). (2) With the increased of concentration and stimulation time of Ox-LDL, the NF-kappaB activity was increased correspondingly and reached the highest when GMCs were stimulated with 50 mg/L Ox-LDL for 4 hours. (3) Supershift assay demonstrated that Ox-LDL mainly activated the two subunits p65 and p50 in GMCs. (4) As the activity of NF-kappaB, O(3m) group vs O(10m) group, O(3m) group vs L(3m) group, and O(10m) group vs L(3m) group had all markedly differences (P<0.01), while the d...Continue Reading

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