Enhanced ability of plasma-activated lactated Ringer's solution to induce A549 cell injury

Archives of Biochemistry and Biophysics
Tatsuki MatsuzakiTetsuo Adachi

Abstract

Non-thermal plasma (NTP) is applicable to living cells and has emerged as a novel technology for cancer therapy. Plasma-activated medium (PAM), which is prepared by the irradiation of culture medium with NTP, induces cell death in cancer cells. However, difficulties are associated with applying PAM to the clinical phase because culture media cannot be used for medical treatments. The objectives of the present study were to demonstrate the inhibitory effects of plasma-activated lactated Ringer's solution (PAL) on the viability of the A549 cancer cell line and elucidate the underlying mechanisms. The anti-tumor activity of PAL was significantly stronger than that of PAM, whereas their concentrations of H2O2 and nitrite were similar. Lactated Ringer's solution (Lac-R) consists of lactate and three types of inorganic salts. The results showing that NTP irradiation of the lactate solution rather than the inorganic salt solution induced the inactivation of catalase were dependent on the presence or absence of nitrite in these solutions. We detected nitrotyrosine in A549 cells treated with PAM or PAL, and the addition of catalase to PAM rather than to PAL reduced its production. The PAL treatment of A549 cells led to mitochondrial dys...Continue Reading

Citations

Jul 23, 2019·Anti-cancer Agents in Medicinal Chemistry·Pankaj Attri, Annemie Bogaerts
Apr 26, 2020·International Journal of Molecular Sciences·Dušan BranýHenrieta Škovierová
Jan 23, 2021·Archives of Biochemistry and Biophysics·Yasumasa OkazakiShinya Toyokuni
May 9, 2021·Archives of Biochemistry and Biophysics·Yasumasa OkazakiShinya Toyokuni
Jan 26, 2022·Free Radical Research·Yasumasa OkazakiShinya Toyokuni

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