PMID: 8594415Jul 19, 1995Paper

Enhanced adhesion of human mononuclear cells to nonenzymatically glycosylated collagen I

Molecular and Cellular Biochemistry
R P Menon, P R Sudhakaran

Abstract

Nonenzymatic glycosylation of extracellular matrix components may contribute to altered interaction of cells with the matrix. We have examined the interaction of mononuclear cells with early glycosylated collagen I. Significantly more cells attached to glycosylated collagen compared to normal collagen. Radioiodinated glycosylated collagen I specifically bound to mononuclear cells in a time and concentration dependent manner with a Kd of 2.45 x 10(9) M. Maximum binding was observed in the presence of Mn++ ions. The iodinated ligand bound to mononuclear cell membrane immobilized on nitrocellulose disks and the interaction was found to be saturable. These results suggested an alteration in the interaction of human blood mononuclear cells with collagen I, when it gets glycosylated non enzymatically and also indicate that early glycosylated collagen interacts with mononuclear cells through specific, high affinity cell surface molecules.

References

May 1, 1992·European Journal of Immunology·R GoldmanN Hogg
Apr 1, 1992·Diabetes Research and Clinical Practice·M Z Gilcrease, R L Hoover
Aug 2, 1990·Nature·T A Springer
Apr 1, 1990·The Journal of Cell Biology·S M AlbeldaC A Buck
Apr 1, 1990·Immunological Reviews·T M Carlos, J M Harlan
Jan 1, 1989·Advances in Immunology·T A Yednock, S D Rosen
Oct 1, 1989·Current Opinion in Cell Biology·S D Rosen
Oct 1, 1987·The Journal of Experimental Medicine·R F BargatzeE C Butcher
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V P PatelH F Lodish

❮ Previous
Next ❯

Citations

Oct 20, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hsin-Jung Wu, Wen-Hsiung Chan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.