Enhanced ALP activity of MG63 cells cultured on hydroxyapatite-poly(ethylene glycol) hydrogel composites prepared using EDTA-OH

Biomedical Materials
Temmei ItoTetsushi Taguchi

Abstract

In order to obtain a hydroxyapatite (HAp)-poly(ethylene glycol) (PEG) composite, tetra amine-terminated PEG was crosslinked using disuccinimidyl tartrate to obtain a PEG hydrogel. Using two kinds of chelators with different stability constants for Ca ion (N-(2-hydroxyethyl) ethylenediamine-N,N',N'-triacetic acid (EDTA-OH, 8.14), and ethylenediamine-N,N,N',N'-tetraacetic acid (EDTA, 10.96)), calcium phosphate was deposited within PEG hydrogels by heating the chelator-containing calcium phosphate solution at 90 °C. X-ray diffraction analysis showed that the deposited calcium phosphate was HAp. The crystallinity of the HAp deposited using EDTA-OH was low compared with that obtained using EDTA, but the amount of HAp deposited within the PEG hydrogel using EDTA-OH was higher than that deposited using EDTA. Significantly more human osteoblast-like MG-63 cells adhered on the HAp-PEG composite prepared using EDTA-OH than on the HAp-PEG composites prepared using EDTA. Furthermore, the alkaline phosphatase activity of MG-63 cultured on the HAp-PEG composite prepared using EDTA-OH was four times higher than that on the HAp-PEG composite prepared using EDTA. Therefore, the HAp-PEG composite prepared using EDTA-OH has potential as a bone su...Continue Reading

References

Jul 4, 2006·Journal of Biomedical Materials Research. Part a·Jinglu ChenBenjamin S Hsiao
Jul 10, 2007·Journal of Biomedical Materials Research. Part a·Avashnee ChettyWim Richter
Nov 14, 2008·Chemical Reviews·Racquel Zapanta LeGeros
Jul 30, 2009·Journal of Materials Science. Materials in Medicine·Tohru HayakawaMitsunobu Sato
Jun 21, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Gabriela IonitaVictor Chechik

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