Enhanced and Complete Removal of Phenylurea Herbicides by Combinational Transgenic Plant-Microbe Remediation

Applied and Environmental Microbiology
Xin YanJiandong Jiang

Abstract

The synergistic relationships between plants and their rhizospheric microbes can be used to develop a combinational bioremediation method, overcoming the constraints of individual phytoremediation or a bioaugmentation method. Here, we provide a combinational transgenic plant-microbe remediation system for a more efficient removal of phenylurea herbicides (PHs) from contaminated sites. The transgenic Arabidopsis thaliana plant synthesizing the bacterial N-demethylase PdmAB in the chloroplast was developed. The constructed transgenic Arabidopsis plant exhibited significant tolerance to isoproturon (IPU), a typical PH, and it took up the IPU through the roots and transported it to leaves, where the majority of the IPU was demethylated to 3-(4-isopropylphenyl)-1-methylurea (MDIPU). The produced intermediate was released outside the roots and further metabolized by the combinationally inoculated MDIPU-mineralizing bacterium Sphingobium sp. strain 1017-1 in the rhizosphere, resulting in an enhanced and complete removal of IPU from soil. Mutual benefits were built for both the transgenic Arabidopsis plant and strain 1017-1. The transgenic Arabidopsis plant offered strain 1017-1 a suitable accommodation, and in return, strain 1017-1 pr...Continue Reading

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Citations

Mar 12, 2019·Frontiers in Microbiology·Shweta JaiswalPratyoosh Shukla
Jun 3, 2021·Applied Microbiology and Biotechnology·Fei YuZhong Hu
May 29, 2019·Journal of Agricultural and Food Chemistry·Junwei HuangJiandong Jiang

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