PMID: 8955419Dec 1, 1996Paper

Enhanced antibiotic production by manipulation of the Streptomyces peucetius dnrH and dnmT genes involved in doxorubicin (adriamycin) biosynthesis

Journal of Bacteriology
C Scotti, C R Hutchinson

Abstract

Sequence analysis of a 3.4-kb region Streptomyces peucetius daunorubicin (DNR) gene cluster established the presence of the dnrH and dnmT genes. In dnrH mutants, DNR production increased 8.5-fold, compared with that in the wild-type strain, while dnmT mutants accumulated epsilon-rhodomycinone (RHO), which normally becomes glycosylated in daunorubicin biosynthesis. Hence, dnmT may be involved in the biosynthesis or attachment of daunosamine to RHO or in the regulation of this process. Since the DnrH protein is similar to known glycosyl transferases, this protein may catalyze the conversion of DNR to its polyglycosylated forms, known as baumycins. Overexpression of dnmT in the wild-type and dnrH mutant strains resulted in a major decrease in RHO accumulation and increase in DNR production.

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Citations

May 29, 2010·Applied Microbiology and Biotechnology·Narayan Prasad NiraulaEung-Soo Kim
Feb 19, 2013·Journal of Basic Microbiology·Ajithkumar VasanthakumarRanjan Prasad
Jan 29, 2008·Chembiochem : a European Journal of Chemical Biology·Carlos OlanoJosé A Salas
Feb 24, 2011·Microbial Biotechnology·Carlos OlanoJosé A Salas
Oct 16, 1999·Glycobiology·D Kapitonov, R K Yu
Oct 13, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Lauren SartorJessica L Vey
Nov 10, 2001·Annual Review of Biomedical Engineering·M KoffasG Stephanopoulos
Jun 5, 2002·Annual Review of Biochemistry·Xuemei M He, Hung-Wen Liu
Nov 18, 1998·Current Opinion in Chemical Biology·D A Johnson, H Liu
Apr 18, 1998·Chemistry & Biology·A M van WageningenP J Solenberg
Jul 28, 2013·Journal of the American Chemical Society·Ahmad Al-MestarihiBrian O Bachmann
Dec 26, 2001·Chemical Reviews·X HeH W Liu Hw
Feb 20, 2002·Chemical Reviews·C. Richard Hutchinson
Oct 5, 2010·Microbiological Research·Bashistha Kumar KanthJae Kyung Sohng

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