Abstract
FILIP1LΔC103 (COOH terminal truncation mutant 1-790 of Filamin A Interacting Protein 1-Like) has been identified to hold therapeutic potential for suppressing tumor growth. Cisplatin (DDP) is commonly used as a first-line drug in the treatment for ovarian cancer. The usage of polymeric nanoparticles to deliver functional genes intraperitoneally holds much promise as an effective therapy for ovarian cancer. In this study, a recombinant plasmid expressing FILIP1LΔC103 (FILIP1LΔC103-p) was constructed, and HPEI nanogels were prepared to deliver FILIP1LΔC103-p into SKOV3 cells. The expression of FILIP1LΔC103 in vitro and in vivo was determined using RT-PCR and Western Blotting. Moreover, in vivo treatment experiments were conducted on nude mice bearing SKOV3 ovarian cancer. The mice were treated with 5% glucose, HPEI+E-p, HPEI+FILIP1LΔC103-p, DDP or HPEI+FILIP1LΔC103-p plus DDP, respectively. Tumor weights were evaluated throughout the treatment duration. The cell proliferation and apoptosis were evaluated by Ki-67 immunochemical staining and TUNEL assay respectively, and the anti-angiogenic effect was assessed by CD31 immunochemical staining and alginate-encapsulated tumor cell assay. FILIP1LΔC103-p could be efficiently transfecte...Continue Reading
References
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·O BoussifJ P Behr
Feb 1, 1994·Gynecologic Oncology·S C MokR S Berkowitz
Jul 30, 1993·Science·S J BrownS J Lippard
Feb 7, 1998·Journal of the Neurological Sciences·N Giladi
Jun 29, 1999·Nature·U M OhndorfS J Lippard
Oct 3, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C J van MoorselG J Peters
Oct 4, 2000·Nature Medicine·Y Q WeiJ Y Liu
Dec 26, 2001·Chemical Reviews·E R Jamieson, S J Lippard
Jan 1, 1997·Apoptosis : an International Journal on Programmed Cell Death·P PeregoF Zunino
Mar 23, 2004·Cell Cycle·Denis R Alexander
Apr 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ryan Jensen, Peter M Glazer
Aug 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Purna MukherjeeThomas N Seyfried
May 28, 2005·The Journal of Gene Medicine·Michael NeuThomas Kissel
Jun 9, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·U LungwitzA Göpferich
Apr 5, 2007·Nature Protocols·Bruno BaudinMichel Vaubourdolle
Sep 17, 2008·Cancer Research·Mijung KwonSteven K Libutti
Apr 24, 2009·Cancer Chemotherapy and Pharmacology·Zhu YuanXia Zhao
Jul 9, 2010·CA: a Cancer Journal for Clinicians·Ahmedin JemalElizabeth Ward
Sep 16, 2010·ACS Nano·MaLing GouYuQuan Wei
Apr 26, 2011·Human Gene Therapy·Chuan XieXia Zhao
Jun 23, 2011·Molecular Cancer Research : MCR·Elizabeth R BurtonMijung Kwon