Enhanced apoptosis of monocytes from complication-free juvenile-onset diabetes mellitus type 1 may be ameliorated by TNF-α inhibitors

Mediators of Inflammation
Jolanta MyśliwskaJanusz Siebert


Diabetes mellitus type 1 is associated with an enhanced apoptosis of different cells and tissues, accelerating occurrence of diabetic microvascular complications. The aim of our study was to determine spontaneous apoptotic potential of the monocyte subsets in juvenile-onset complication-free diabetes mellitus type 1 and to compare them with the corresponding values of the healthy. Moreover, we wanted to assess effects of TNF-R1 blocking agents and those of general TNF-α blocker (Infliximab) on spontaneous apoptosis of monocytes. Sixty randomly selected DM1 patients (14.5 ± 3.2 years) and 30 healthy (13.5 ± 2.8 years) volunteers were enrolled in the study. Our results indicate that three monocyte subsets are distinguishable in the groups of young diabetic patients and the healthy, similarly to in the blood of adults. DM1 patients were characterized by higher values of apoptotic monocytes than the healthy. The manipulation with drugs inhibiting TNF-R1 expression diminished the pool of CD16(+) apoptotic monocytes. Infliximab reduced the apoptotic CD16(-) cells. In conclusion, diabetes mellitus type 1 is associated with greater apoptosis of three monocyte subsets which may contribute to the development of microvascular complication...Continue Reading


Feb 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Antonia M JoussenAnthony P Adamis
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kai-Uwe BelgeLöms Ziegler-Heitbrock
Jul 11, 2002·European Journal of Pharmacology·Rainer Amann, Bernhard A Peskar
Jan 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stephen A RenshawMoira K B Whyte
Dec 11, 2003·Journal of Molecular Endocrinology·W M KühtreiberDenise Faustman
Jul 17, 2004·Neoplasia : an International Journal for Oncology Research·Paola SecchieroGiorgio Zauli
Jun 10, 2005·Journal of Biochemistry and Molecular Biology·Liv Thommesen, Astrid Laegreid
Nov 15, 2006·Clinical and Experimental Immunology·E HatanakaA Campa
Jun 26, 2007·Inflammation·James L MobleyRichard Renkiewicz
Sep 4, 2007·Acta Diabetologica·Yuji MoriwakiTetsuya Yamamoto
Jan 13, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ellen C Ebert
Feb 19, 2009·Biochemical and Biophysical Research Communications·Cong-Yi WangFeili Gong
Aug 20, 2009·Immunology·Tomasz P MikołajczykJuliusz R Pryjma
May 22, 2010·Nature Reviews. Drug Discovery·Denise Faustman, Miriam Davis
Jul 16, 2010·Blood·Loems Ziegler-HeitbrockManfred B Lutz
Mar 3, 2011·The American Journal of Gastroenterology·Sergio RutellaSilvio Danese
Apr 13, 2011·Molecular Immunology·Paola I IngaramoCristina E Carnovale
Jun 10, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Dong-Sub JungShin-Wook Kang
Aug 4, 2011·American Journal of Physiology. Endocrinology and Metabolism·Isamu YamakawaHiroshi Kimura
Feb 22, 2012·Middle East African Journal of Ophthalmology·Sampathkumar RangasamyArup Das
Mar 21, 2012·Immunologic Research·Kok Loon WongSiew Cheng Wong
Sep 4, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gaetano ZizzoPhilip L Cohen
Jan 15, 2013·Mediators of Inflammation·Danqing MinSusan V McLennan
Jan 26, 2013·PloS One·Stephan DreschersThorsten W Orlikowsky


Jun 14, 2015·Archivos de bronconeumología·Aysel KurtAhmet Acipayam
Jul 23, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Monika Ryba-StanisławowskaMałgorzata Myśliwiec
Dec 17, 2014·Cellular & Molecular Immunology·Cecilia Analia PanekMarina Sandra Palermo
Jun 22, 2017·Molecular and Cellular Biochemistry·Bartosz SłomińskiMałgorzata Myśliwiec

Methods Mentioned

density gradient centrifugation
Flow Cytometry

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