Enhanced arachidonic acid metabolism and human neutrophil migration in asthma

Prostaglandins, Leukotrienes, and Essential Fatty Acids
T RadeauP Godard

Abstract

In stable state asthmatic patients (AP) without any airway obstruction, the capacity of peripheral blood polymorphonuclear neutrophils (PMN) to produce 5-lipoxygenase metabolites and to migrate, was investigated and compared with the response in healthy subjects (HS). After calcium-ionophore A23187 stimulation, PMN from AP and HS produced LTB4, its hydroxylated derivatives: omega-OH-and omega-CO2H-LTB4) (omega-LTB4, i.e 6-trans-LTB4 and 5,6-diHETE isomers, and 5-HETE. We found an increase in LTB4 (+59%), omega-LTB4 (+39%), 6-trans-LTB4 (+128%), and free 5-HETE (+63%) generation of AP as compared with HS. Unstimulated migration was enhanced in AP (122 +/- 27 PMN/10 high power fields (hpf) in AP versus 74 +/- 25 PMN/10 hpf in HS, p less than 0.025) and suggested a greater capacity of PMN from AP to migrate. This was confirmed by the PAF-induced chemotaxis studies which showed, in AP, a greater PAF-sensitivity of PMN (10(-6) M versus 10(-5) M in HS) and a greater chemotaxis response (600 +/- 50 PMN versus 200 +/- 35 PMN in HS). In AP, we compared the capacity of PMN to generate LTB4 and 5-HETE with their capacity to migrate. We found an inverse correlation (r = 0.86, p less than 0.007) of intracellular free 5-HETE with chemotaxis ...Continue Reading

References

May 1, 1989·The Journal of Allergy and Clinical Immunology·S MeltzerJ Easton
Aug 1, 1986·Pharmacological Research Communications·C P Page, J Morley
Feb 1, 1985·The Journal of Allergy and Clinical Immunology·M P CarrollA B Kay
Jan 1, 1984·The Journal of Investigative Dermatology·H Mensing, B M Czarnetzki
Mar 12, 1984·FEBS Letters·J VerhagenJ F Vliegenthart
Feb 28, 1983·Biochemical and Biophysical Research Communications·J T O'FlahertyR L Wykle
Dec 1, 1983·The Journal of Allergy and Clinical Immunology·B R O'DriscollA B Kay

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Citations

Mar 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·E ThomasC Chavis
May 2, 2003·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Sheldon L Spector, Marc E Surette
Jun 25, 2015·Immunological Investigations·Tainá MoscaWilma C N Forte
Oct 9, 2009·Current Medical Research and Opinion·Julianne LindemannDean Stull
Jun 11, 1999·Analytical Biochemistry·C ChavisJ Bousquet
Sep 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A P Sampson

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