Enhanced Autophagy Contributes to Protective Effects of GM1 Ganglioside Against Aβ1-42-Induced Neurotoxicity and Cognitive Deficits

Neurochemical Research
Ruwei DaiQi Wang

Abstract

Alzheimer's disease (AD) is a progressive neurodegenerative disorder. The aggregation of Aβ peptides, Aβ1-42 in particular, is thought to be a fundamental pathogenic mechanism leading to the neuronal damage in AD. Recently, monosialoganglioside GM1 is reported to possess pivotal neuroprotection in neurodegenerative diseases. Previous studies have focused on the conformational dynamics and the biochemical interaction of the amyloid-peptide with the GM1 ganglioside, as well as the protective effect of GM1 on cognition. However, the phenomenon of autophagy with regard to neuronal dysfunction in AD is less investigated. In the present study, GM1 treatment were investigated in an AD mouse model and cultured PC12 dells to examine cognition-protective and neuroprotective effects of GM1. Furthermore, GM1 was found to induce autophagy via testing light chain 3 (LC3), Beclin1, neighbor of BRCA1 gene 1 protein and p62 (a substrate of LC3). Chloroquine, an inhibitor of lysosomal, was used to exclude the interference of lysosome, which could fuse with autophagosome and then clear it. In the presence of the inhibitor of autophagy (3-methyladenine; 3-MA), the protective effect of GM1 on PC12 cells in Aβ (1-42) induced toxic conditions was dim...Continue Reading

References

Aug 31, 2000·Annual Review of Biochemistry·J Kim, D J Klionsky
Sep 10, 2002·Dementia and Geriatric Cognitive Disorders·Lars SvennerholmCarsten Wikkelsö
Dec 5, 2002·Current Opinion in Structural Biology·Maria Laura Allende, Richard L Proia
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zhenyu YueNathaniel Heintz
Apr 23, 2004·Trends in Cell Biology·Ana Maria Cuervo
Nov 6, 2004·Science·Takahiro Shintani, Daniel J Klionsky
Aug 12, 2006·The Journal of Biological Chemistry·Tomohiro YorimitsuDaniel J Klionsky
Jul 27, 2007·Cell Biochemistry and Biophysics·Klaus Beyer
Aug 23, 2007·Nature Reviews. Molecular Cell Biology·Daniel J Klionsky
Mar 13, 2008·Journal of Lipid Research·Toshio ArigaRobert K Yu
Mar 10, 2009·Autophagy·Shih-Ya HungWen-Mei Fu
Mar 25, 2009·Trends in Neurosciences·C Savio ChanD James Surmeier
Dec 17, 2009·Brain : a Journal of Neurology·Fiona M MenziesDavid C Rubinsztein
Jan 5, 2010·European Journal of Pharmacology·Analía ValdomeroOtto A Orsingher
Jun 21, 2012·Journal of Neuroscience Research·Gusheng WuRobert W Ledeen
Jan 5, 2013·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Meige ZhengGuoying Li
May 4, 2013·Cell Death & Disease·Longfei DengYuquan Wei
May 10, 2013·The New England Journal of Medicine·Andrea DoriaLeonardo Punzi
Nov 14, 2013·Biochemical and Biophysical Research Communications·Kun-Peng LiuXian-Hui He
Feb 8, 2014·Behavioural Brain Research·Emma L HarrisonBernhard T Baune
Jan 21, 2015·Biochimica Et Biophysica Acta·Tomotake KankiShun-ichi Yamashita
Jul 26, 2015·Biochemical and Biophysical Research Communications·Jeong Su ParkSoo Han Bae
Jun 14, 2016·Angewandte Chemie·Mariana AmaroMartin Hof

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Citations

Jun 17, 2018·Biological Chemistry·Gerhild van Echten-Deckert, Shah Alam
Oct 11, 2017·EMBO Molecular Medicine·Melanie AlpaughSimonetta Sipione
Jan 16, 2020·Molecular Medicine Reports·Xiaopeng Wang, Jing Zhao
Aug 27, 2019·Frontiers in Neurology·Pierre J MagistrettiSimonetta Sipione
Sep 12, 2018·Journal of Molecular Neuroscience : MN·Na LuHaixia Lu
Jan 13, 2018·International Journal of Molecular Medicine·Hua JiLihui Wu
Apr 25, 2020·International Journal of Molecular Sciences·Erika Di BiaseElena Chiricozzi
Feb 2, 2021·Frontiers in Neuroscience·Henriette Haukedal, Kristine K Freude
Oct 30, 2020·Frontiers in Neuroscience·Simonetta SipioneVaibhavi Kadam
May 16, 2021·Brain Research Bulletin·Kangkang YangWenzhe Li

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Methods Mentioned

BETA
ELISA
transfection
acetylation
histone acetylation

Software Mentioned

Statistics Package for Social Science ( SPSS )
Quantity One Analysis

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