Enhanced Biocompatibility of Multi-Layered, 3D Bio-Printed Artificial Vessels Composed of Autologous Mesenchymal Stem Cells

Polymers
Eui Hwa JangYoung-Nam Youn

Abstract

Artificial vessels capable of long-term patency are essential clinical tools in vascular surgery that involves small vessels. On-going attempts to develop artificial vessels that complements restenosis have not been entirely successful. Here, we report on the fabrication of small-sized artificial vessels using a three-dimensional bio-printer. The fabrication employed biodegradable polycaprolactone and autologous MSCs harvested from the bone-marrow of canines. The MSCs were cultured and differentiated into endothelial-like cells. After confirming differentiation, artificial vessels comprising three-layers were constructed and implanted into the arteries of canines. The autologous MSCs printed on artificial vessels (cell-derived group) maintained a 64.3% patency (9 of 14 grafts) compared with artificial vessels without cells (control group, 54.5% patency (6 of 11 grafts)). The cell-derived vessels (61.9 cells/mm2 ± 14.3) had more endothelial cells on their inner surfaces than the control vessels (21 cells/mm2 ± 11.3). Moreover, the control vessels showed acute inflammation on the porous structures of the implanted artificial vessels, whereas the cell-derived vessels exhibited fibrinous clots with little to no inflammation. We con...Continue Reading

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Citations

Oct 10, 2020·Frontiers in Cardiovascular Medicine·Peter ZillaTim Pennel
Jun 8, 2021·JACC. Basic to Translational Science·Kevin SungKim-Lien Nguyen
Jun 9, 2021·Biofabrication·Faraz FazalNorbert Radacsi
Oct 12, 2021·Veterinary Surgery : VS·Johnny AltwalDominique J Griffon

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Methods Mentioned

BETA
Protein Assay
scanning electron microscopy
light microscopy

Software Mentioned

Image J
SPSS

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