Enhanced biomass and oil production from sugarcane bagasse hydrolysate (SBH) by heterotrophic oleaginous microalga Chlorella protothecoides

Bioresource Technology
Jinxiu MuYuqin Li

Abstract

The potential use of sugarcane bagasse hydrolysate (SBH) for microalgal oil in a heterotrophic mode and the oil accumulation mechanisms by SBH-induced Chlorella protothecoides cells were investigated in this study. Results demonstrated that SBH performed better than glucose for cell growth and lipid accumulation under the same reducing sugar concentration. The lipid productivity of 0.69g/L/d was accomplished at 40g/L of reducing sugar by batch culture. Under the fed-batch culture condition, the maximum biomass and lipid productivity were 24.01g/L and 1.19g/L/d, respectively. Metabolic pathway analysis results indicated that xylose and arabinose involved in pentose phosphate pathway might be predominant over sole glucose involved in glycolysis for lipid accumulation in cells. Three metabolic checkpoints in the proposed metabolic network, including xylulose kinase, acyl-CoA dehydrogenase, and dihydrolipoyl dehydrogenase reveal new possibilities in developing genetic and metabolic engineering microalgae for desirable lipid productivity.

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Citations

Mar 8, 2018·Critical Reviews in Biotechnology·Jiaxin ChenWenyi Dong
Sep 13, 2018·Recent Patents on Food, Nutrition & Agriculture·Mohammad H Morowvat, Younes Ghasemi
Dec 5, 2019·Brazilian Journal of Biology = Revista Brasleira De Biologia·L H Sipaúba-TavaresM G Tedesque
Feb 2, 2017·Frontiers in Bioengineering and Biotechnology·Calvin A HenardEric P Knoshaug
Sep 18, 2020·Letters in Applied Microbiology·L YinR Q Huang
Jan 9, 2021·Bioresource Technology·E Tomás-PejóC González-Fernández
Sep 4, 2021·Frontiers in Microbiology·Anjani Devi ChintaguntaJesus Simal-Gandara

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