Abstract
The potential use of sugarcane bagasse hydrolysate (SBH) for microalgal oil in a heterotrophic mode and the oil accumulation mechanisms by SBH-induced Chlorella protothecoides cells were investigated in this study. Results demonstrated that SBH performed better than glucose for cell growth and lipid accumulation under the same reducing sugar concentration. The lipid productivity of 0.69g/L/d was accomplished at 40g/L of reducing sugar by batch culture. Under the fed-batch culture condition, the maximum biomass and lipid productivity were 24.01g/L and 1.19g/L/d, respectively. Metabolic pathway analysis results indicated that xylose and arabinose involved in pentose phosphate pathway might be predominant over sole glucose involved in glycolysis for lipid accumulation in cells. Three metabolic checkpoints in the proposed metabolic network, including xylulose kinase, acyl-CoA dehydrogenase, and dihydrolipoyl dehydrogenase reveal new possibilities in developing genetic and metabolic engineering microalgae for desirable lipid productivity.
References
Jun 15, 2006·Journal of Biotechnology·Han XuQingyu Wu
Jun 30, 2006·Archives of Biochemistry and Biophysics·Neeti Sanan MishraNarendra Tuteja
Jun 30, 2007·Microbiology·Ying ZhangColin Ratledge
Apr 9, 2008·Bioresource Technology·L Y ZhuH Wu
Jul 11, 2008·Bioresource Technology·Emily R EasterlingMargarita Licha
Aug 12, 2008·Applied Microbiology and Biotechnology·Qiang LiDehua Liu
Jul 28, 2009·Journal of Industrial Microbiology & Biotechnology·Aili WeiShang-Tian Yang
Jun 29, 2010·Phytochemistry·Chang-Xia DuJuan Li
Aug 17, 2010·Journal of Industrial Microbiology & Biotechnology·Xin ZhaoZongbao K Zhao
Aug 20, 2010·Plant Physiology·Wei XiongQingyu Wu
Jul 16, 2011·Bioresource Technology·Yeshitila Asteraye TsigieYi-Hsu Ju
Mar 13, 2012·Bioresource Technology·Jianhua FanShulan Li
Sep 11, 2012·Biotechnology Advances·Chao HuangYong Chen
Sep 27, 2012·Bioresource Technology·Rongming LiuPingkai Ouyang
Jun 12, 2013·Journal of Proteomics·Michael T GuarnieriPhilip T Pienkos
Jan 7, 2014·Bioresource Technology·Alessandro ConcasGiacomo Cao
Citations
May 24, 2015·Bioresource Technology·Junhui ChenGu Chen
Oct 29, 2015·Bioresource Technology·Zhangzhang XieJianfei Luo
Apr 10, 2016·Bioresource Technology·Di CaiTianwei Tan
Apr 21, 2016·Applied Biochemistry and Biotechnology·Neha AroraVikas Pruthi
Sep 14, 2016·Bioresource Technology·Shikai WangXu Wang
Mar 8, 2018·Critical Reviews in Biotechnology·Jiaxin ChenWenyi Dong
May 3, 2019·Scientific Reports·Minsik KimYong Keun Chang
Jun 28, 2018·Biotechnology for Biofuels·Alok PatelPaul Christakopoulos
Sep 13, 2018·Recent Patents on Food, Nutrition & Agriculture·Mohammad H Morowvat, Younes Ghasemi
Dec 5, 2019·Brazilian Journal of Biology = Revista Brasleira De Biologia·L H Sipaúba-TavaresM G Tedesque
Mar 29, 2019·Bioprocess and Biosystems Engineering·Jun-Hui ChenDong Wei
Jan 23, 2018·Applied Microbiology and Biotechnology·Ben ZhaoWu Wang
Jul 8, 2019·World Journal of Microbiology & Biotechnology·Zhenyao WangYuqin Li
May 6, 2020·Environmental Science and Pollution Research International·Mei AnMing Li
Feb 2, 2017·Frontiers in Bioengineering and Biotechnology·Calvin A HenardEric P Knoshaug
Mar 6, 2019·Scientific Reports·Chiara LauritanoAdrianna Ianora
Sep 18, 2020·Letters in Applied Microbiology·L YinR Q Huang
Jan 9, 2021·Bioresource Technology·E Tomás-PejóC González-Fernández
Sep 4, 2021·Frontiers in Microbiology·Anjani Devi ChintaguntaJesus Simal-Gandara
Oct 21, 2021·Biotechnology Journal·Minsik KimYong Keun Chang