Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold

International Journal of Nanomedicine
Xing WangBin Zhao

Abstract

Insulin is widely considered as a classical hormone and drug in maintaining energy and glucose homeostasis. Recently, insulin has been increasingly recognized as an indispensable factor for osteogenesis and bone turnover, but its applications in bone regeneration have been restricted because of the short periods of activity and uncontrolled release. In this study, we incorporated insulin-loaded poly lactic-co-glycolic-acid (PLGA) nanospheres into nano-hydroxyapatite/collagen (nHAC) scaffolds and investigated the bioactivity of the composite scaffolds in vitro and in vivo. Bioactive insulin was successfully released from the nanospheres within the scaffold, and the release kinetics of insulin could be efficiently controlled by uniform-sized nanospheres. The physical characterizations of the composite scaffolds demonstrated that incorporation of nanospheres in nHAC scaffolds using this method did not significantly change the porosity, pore diameters, and compressive strengths of nHAC. In vitro, the insulin-loaded nHAC/PLGA composite scaffolds possessed favorable biological function for bone marrow mesenchymal stem cells adhesion and proliferation, as well as the differentiation into osteoblasts. In vivo, the optimized bone regene...Continue Reading

Citations

Aug 21, 2020·Advanced Healthcare Materials·Behnoush KhorsandAliasger K Salem
Dec 8, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhimin XuBing Han
Aug 31, 2020·Laryngoscope Investigative Otolaryngology·Weston L NiermeyerTendy Chiang
Feb 6, 2020·Soft Matter·Wenshuai LiuWeiwei Wang
Apr 3, 2021·Npj Regenerative Medicine·Julia LosnerJessica L Whited
May 6, 2019·International Journal of Biological Macromolecules·Mojtaba AbbasianMehdi Jaymand
Aug 22, 2021·Calcified Tissue International·Joseph BidwellCarmella Evans-Molina

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Methods Mentioned

BETA
protein assay
enzyme-linked immunosorbent assay
ELISA
Assay

Software Mentioned

MATLAB
BioQuant
SPSS

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