Enhanced Boron Tolerance in Plants Mediated by Bidirectional Transport Through Plasma Membrane Intrinsic Proteins

Scientific Reports
Kareem A MosaOm Parkash Dhankher

Abstract

High boron (B) concentration is toxic to plants that limit plant productivity. Recent studies have shown the involvement of the members of major intrinsic protein (MIP) family in controlling B transport. Here, we have provided experimental evidences showing the bidirectional transport activity of rice OsPIP1;3 and OsPIP2;6. Boron transport ability of OsPIP1;3 and OsPIP2;6 were displayed in yeast HD9 mutant strain (∆fps1∆acr3∆ycf1) as a result of increased B sensitivity, influx and accumulation by OsPIP1;3, and rapid efflux activity by OsPIP2;6. RT-PCR analysis showed strong upregulation of OsPIP1;3 and OsPIP2;6 transcripts in roots by B toxicity. Transgenic Arabidopsis lines overexpressing OsPIP1;3 and OsPIP2;6 exhibited enhanced tolerance to B toxicity. Furthermore, B concentration was significantly increased after 2 and 3 hours of tracer boron ((10)B) treatment. Interestingly, a rapid efflux of (10)B from the roots of the transgenic plants was observed within 1 h of (10)B treatment. Boron tolerance in OsPIP1;3 and OsPIP2;6 lines was inhibited by aquaporin inhibitors, silver nitrate and sodium azide. Our data proved that OsPIP1;3 and OsPIP2;6 are indeed involved in both influx and efflux of boron transport. Manipulation of the...Continue Reading

References

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Citations

Mar 26, 2016·Frontiers in Plant Science·Kareem A MosaOm Parkash Dhankher
Aug 3, 2016·International Journal of Molecular Sciences·Min WangShiwei Guo
Sep 8, 2016·Proceedings of the National Academy of Sciences of the United States of America·Bryan H Thurtle-Schmidt, Robert M Stroud
Oct 16, 2016·Plant, Cell & Environment·Michael GroszmannJohn R Evans
Dec 6, 2017·Frontiers in Plant Science·Akira Yoshinari, Junpei Takano
Mar 31, 2018·Frontiers in Plant Science·Xiaofen YuGuangyuan He
Jun 27, 2019·Planta·Marco LandiFabrizio Araniti

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Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

PrimerQuest

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