Enhanced branching morphogenesis in mammary glands of mice lacking cell surface beta1,4-galactosyltransferase

Developmental Biology
Kristin SteffgenHelen Hathaway

Abstract

Development of the mammary gland is influenced both by the systemic hormonal environment and locally through cell-cell and cell-extracellular matrix (ECM) interactions. We have previously demonstrated aberrant mammary gland morphogenesis in transgenic mice with elevated levels of the long isoform of beta1,4-galactosyltransferase 1 (GalT), a proportion of which is targeted to the plasma membrane, where it plays a role in cell-ECM interactions. Here, we show that mammary glands of mice lacking the long GalT isoform exhibit a complementary phenotype. Cell-surface GalT activity was reduced by over 60%, but because the short GalT isoform is intact, total GalT activity was reduced only slightly relative to wild type. Mammary glands from long GalT-null mice were characterized by excess branching, and this phenotype was accompanied by altered expression of laminin chains. Laminin alpha1 and alpha3 were reduced 2.4- and 3.0-fold, respectively, while expression of laminin gamma2 was elevated 2.3-fold. The expression and cleavage of laminin gamma2 have been correlated with branching and cell migration, and Western blotting revealed an altered pattern in gamma2 cleavage products in long GalT-null mammary glands. We then examined the expres...Continue Reading

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Citations

Nov 8, 2006·Journal of Mammary Gland Biology and Neoplasia·Jillian HowlinFinian Martin
Nov 6, 2002·Biochimica Et Biophysica Acta·Carey Rodeheffer, Barry D Shur
Jun 12, 2010·Journal of Biochemistry·Yuanyan WeiJianhai Jiang
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May 5, 2020·Glycobiology·Krzysztof MikolajczykMarcin Czerwinski
Oct 13, 2005·Journal of Cell Science·Eric C DietzeVictoria L Seewaldt

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