Enhanced catharanthine production in catharanthus roseus cell cultures by combined elicitor treatment in shake flasks and bioreactors

Enzyme and Microbial Technology
J ZhaoQ Hu

Abstract

Chemical and fungal elicitors were added to Catharanthus roseus cell suspension cultures so as to improve the production of indole alkaloids. A synergistic effect on alkaloid accumulation was observed in C. roseus cell cultures when treated with some combined elicitors of fungal preparations and chemicals. Among them, the combination of tetramethyl amminium bromide and Aspergillum niger mycelial homogenate gave the highest ajmalicine yield (63 mg l(-1)) and an improved catharanthine accumulation (17 mg l(-1)). The combined elicitors of malate and sodium alginate resulted in the highest catharanthine yield (26 mg l(-1)) and a high ajmalicine accumulation (41 mg l(-1)) in the cell cultures. Based on the synergistic effect of malate and sodium alginate, a process with enhanced catharanthine production in Catharanthus roseus cell cultures was developed in shake flasks and a bioreactor. After 10 days of culture, 25 mg l(-1), 32 mg l(-1) and 22 mg l(-1) catharanthine yield were obtained in 500-ml flasks, 1000-ml flasks and in a 20-l airlift bioreactor, respectively. Upon malate-alginate combining treatments, peroxidase, catalase and superoxide dismutase activities decreased in elicited cells but phenylalanine ammonia lyase and lipoxy...Continue Reading

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Citations

Apr 7, 2011·Applied Microbiology and Biotechnology·Mei-Liang ZhouYan-Min Wu
May 19, 2011·Applied Microbiology and Biotechnology·Mei-Liang ZhouYi-Xiong Tang
Dec 21, 2013·Applied Biochemistry and Biotechnology·Smita Srivastava, A K Srivastava
Jan 23, 2014·Applied Biochemistry and Biotechnology·Pratibha GuptaSanjay Saxena
Mar 1, 2012·Journal of Pharmacy & Bioallied Sciences·Md Sarfaraj HussainMohd Saeed
Jan 8, 2016·Plant Physiology and Biochemistry : PPB·Lorena AlmagroM A Pedreño
Jul 22, 2010·Pharmaceutical Biology·S Rahimi AshtianiM R Bihamta
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Feb 17, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lorena AlmagroMaria Angeles Pedreño
Apr 19, 2020·Applied Microbiology and Biotechnology·Anamika DasSaikat Gantait
Apr 26, 2005·Biotechnology Advances·Jian ZhaoRobert Verpoorte

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