Enhanced cellular responses of human bone marrow stromal cells cultured on pretreated surface with allogenic platelet-rich plasma

Connective Tissue Research
Seung Han ShinHee Joong Kim

Abstract

The principal objective of this study was to evaluate the effects of surface pretreatment with platelet-rich plasma (PRP) on the cellular functions of human bone marrow stromal cells (hBMSCs). The surfaces of tissue culture plates (TCPs) were pretreated by adding PRP followed by centrifugation to bring platelets closer to the surface, followed by incubation for 60 min at 37°C. Then, hBMSCs were seeded onto TCP and TCP pretreated with PRP (TCP-PRP), followed by culture in osteogenic medium. Cell attachment, proliferation, and osteogenic differentiation were evaluated. Field emission scanning electron microscope (FE-SEM; JSM-7401F, JEOL Ltd., Japan) observations were conducted. The attachment of hBMSCs was significantly lower on TCP-PRP than on TCP. However, when the cell numbers were normalized with those observed on day 1 of culture, cellular proliferation on 5 days was significantly higher on TCP-PRP. Alkaline phosphatase activity, an index of early phase of osteoblastic differentiation, was significantly higher on TCP-PRP on day 14. Calcium deposition amount, an index of terminal osteoblastic differentiation, was also significantly higher on TCP-PRP on days 14 and 21. The results of von Kossa staining confirmed that, on day 2...Continue Reading

References

Mar 1, 1993·Blood Reviews·P Harrison, E M Cramer
Jan 1, 1996·International Review of Cytology·C J Schaffer, L B Nanney
Feb 1, 1997·Journal of Cellular Biochemistry·N JaiswalS P Bruder
Jun 25, 1998·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·R E MarxK R Georgeff
Jan 23, 1999·Tissue Engineering·A M MackayM F Pittenger
Apr 2, 1999·Science·M F PittengerD R Marshak
Aug 22, 2000·Journal of Biomedical Materials Research·M S PonticielloF P Barry
Feb 24, 2001·The New England Journal of Medicine·R QuartoM Marcacci
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yang D TengEvan Y Snyder
Jul 30, 2002·Mund-, Kiefer- und Gesichtschirurgie : MKG·G WeibrichW Wagner
Sep 17, 2002·Calcified Tissue International·B D BoyanA L Boskey
Aug 5, 2003·Biomaterials·Enrico LucarelliPiero Picci
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Cecilia ErikssonLars Oster
Oct 18, 2003·The Journal of Bone and Joint Surgery. American Volume·Treena Livingston ArinzehSudha Kadiyala
Dec 16, 2003·Birth Defects Research. Part C, Embryo Today : Reviews·Frank P Barry
Mar 30, 2004·The Journal of Bone and Joint Surgery. British Volume·A VatsJ M Polak
Aug 5, 2004·Journal of Biomedical Materials Research. Part a·S LossdörferB D Boyan
Oct 27, 2004·Anesthesia and Analgesia·Jun KawasakiKenichi A Tanaka
Mar 2, 2005·Tissue Engineering·Cameron J WilsonMark J Pearcy
Mar 3, 2005·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Takahiro KannoTatsuji Nishihara
May 12, 2005·Journal of Cellular Physiology·Christelle DoucetJean-Jacques Lataillade
Aug 25, 2005·Clinical Oral Implants Research·Cimara Fortes FerreiraRicardo de Souza Magini
Aug 27, 2005·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Yoichiro OginoKiyoshi Koyano
Mar 17, 2006·Trends in Biotechnology·Eduardo AnituaIsabel Andía
Apr 13, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·D DallariA Giunti
Nov 14, 2006·The American Journal of Sports Medicine·Mikel SánchezIñigo Mujika
Sep 18, 2007·Biochemical and Biophysical Research Communications·Yu-Ming LiRegina Ebert
Feb 20, 2008·International Orthopaedics·Jeong Joon YooHee Joong Kim
Jul 3, 2008·Journal of Orthopaedic Trauma·Samir Mehta, J Tracy Watson

❮ Previous
Next ❯

Citations

May 7, 2013·Trends in Biotechnology·Eduardo AnituaGorka Orive
Sep 13, 2013·Therapeutic Delivery·Eduardo AnituaGorka Orive
Jul 24, 2012·Revue de stomatologie et de chirurgie maxillo-faciale·G Bettega, E Schir
Jun 20, 2014·Journal of Tissue Engineering and Regenerative Medicine·Hong Suk KwakJeong Joon Yoo
May 21, 2014·The Open Orthopaedics Journal·Steven Elder, John Thomason
Aug 5, 2016·Tissue Engineering and Regenerative Medicine·Ji-Hye LeeJeong Joon Yoo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons
Bahadir GürbüzerFeriha Ercan
Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research
Roberto S PessoaDenildo de Magalhaes
© 2022 Meta ULC. All rights reserved