Enhanced chemiluminescence detection of glutathione based on isoluminol-PSM nanoparticles probe

Talanta
Xiaoting JiCaifeng Ding

Abstract

In this paper, a new chemiluminescence (CL) strategy was constructed for the determination of physiological thiols by using an isoluminol labeled nano-probe. The amino group on the surface of the magnetic beads (MBs) were converted into pyridyl disulfide groups by treatment with N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP), in the meantime, isoluminol and thiolated signal DNA were labeled on the surface of the polystyrene microspheres (PSMs). By treating the SPDP activated MBs with the modified PSMs, isoluminol molecules on the surface of the PSMs, along with the thiolated signal DNA, were attached to the surface of the MBs through disulfide bonds to form a CL probe. In the presence of glutathione (GSH), the disulfide bonds could be cleaved readily. The isoluminol molecules modified on the surface of the PSMs released from the CL probes were detached by magnetic separation and transferred to the dark closet for CL detection of isoluminol-H2O2-HRP system. Using GSH detection as a model, we prove a linear dose response in the range from 5 × 10(-10) to 8 × 10(-8)M. The detection limit of this trial for GSH determined is 5 × 10(-10)M. The proposed design was successfully applied to the extracts of K562 cell for intracellular...Continue Reading

References

Oct 6, 1995·Journal of Chromatography. B, Biomedical Applications·W A Kleinman, J P Richie
Nov 13, 2004·Journal of the American Chemical Society·Akimitsu OkamotoIsao Saito
Nov 1, 2006·Analytical Chemistry·Eden Joy Pacsial-OngRobert M Strongin
Mar 5, 2008·Journal of the American Chemical Society·Jinhao GaoBing Xu
Feb 6, 2010·The Journal of Physical Chemistry. B·Raquel MaeztuGustavo González-Gaitano
Aug 13, 2010·The Journal of Physical Chemistry. B·Raquel MaeztuGloria Tardajos
Jan 21, 2014·Analytical and Bioanalytical Chemistry·Sihua Qian, Hengwei Lin
Mar 29, 2014·Journal of the American Chemical Society·Yong-Siou Chen, Prashant V Kamat

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Citations

Apr 29, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qianru MaYufen Zhao
Jul 13, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Cailing JiangLi Jiang
Sep 7, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mohamed Ibrahim HalawaBing Shi Li

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