Enhanced clearance of Escherichia coli and Staphylococcus aureus in mice treated with cyclophosphamide and lactoferrin

International Immunopharmacology
J ArtymMarian L Kruzel

Abstract

Previous studies on cyclophosphamide (CP)-immunocompromised mice showed accelerated reconstitution of immune system function following oral treatment with lactoferrin (LF). The aim of this investigation was to evaluate the ability of mice, treated with a sublethal dose of CP and given LF, to combat bacterial infections. Mice were injected with a single, intraperitoneal dose of CP (350 mg/kg body weight). One group of CP-treated mice was also given LF in drinking water (0.5% solution) for 14 days. Untreated and LF-treated mice served as controls. On day 15 following CP administration, mice were infected intravenously with 10(8) Escherichia coli or 5 x 10(7) Staphylococcus aureus. Twenty-four hours later, the number of colony-forming units (CFU) in spleens and livers were determined. Phenotypic analysis of blood leukocytes was determined, as well as the ability of splenic and peritoneal cells to produce IL-6 spontaneously and in the presence of lipopolysaccharide (LPS). Treatment with CP, or with CP and LF, led to profound reduction of E. coli CFU in the liver and the spleen; treatment with LF alone had significant inhibitory effects on organ enumerated CFU. S. aureus CFUs were also significantly reduced in spleens of mice treate...Continue Reading

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Citations

Jun 18, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Christie G TurinUNKNOWN NEOLACTO Research Group
Oct 10, 2009·Pharmacological Reports : PR·Michał ZimeckiMaja Kocieba
Mar 13, 2014·International Immunopharmacology·Shen-An HwangJeffrey K Actor
Jan 14, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Theresa J Ochoa, Stéphane V Sizonenko
Mar 4, 2017·International Journal of Molecular Sciences·Maria Elisa Drago-SerranoMireya de la Garza
Nov 23, 2017·Frontiers in Immunology·Marian L KruzelJeffrey K Actor
Jun 3, 2009·Expert Review of Anti-infective Therapy·Mohan Venkatesh, Steven Abrams
Jul 25, 2013·Expert Review of Anti-infective Therapy·Krithika LingappanMohan Pammi
Jun 29, 2017·The Cochrane Database of Systematic Reviews·Mohan Pammi, Gautham Suresh

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