PMID: 7537958May 1, 1995Paper

Enhanced complement susceptibility of avidin-biotin-treated human erythrocytes is a consequence of neutralization of the complement regulators CD59 and decay accelerating factor

The Biochemical Journal
A B ZaltzmanB P Morgan

Abstract

Biotinylation of erythrocytes (E) followed by avidin cross-linking at specific sites has been suggested as a novel means of drug delivery. Upon avidin cross-linking, biotinylated E become complement-activating and highly susceptible to complement lysis, thus bringing about release of entrapped drug. We set out to examine the mechanisms of this biotin-avidin-induced lytic susceptibility, focusing on the effects of biotinylation and avidin cross-linking on the major E complement regulatory molecules, decay accelerating factor (DAF) and CD59. We demonstrate here that biotinylation of E, which does not render them complement activating, partially inhibits DAF but has little effect on CD59. Subsequent cross-linking with avidin causes complete inhibition of DAF and near complete loss of CD59 activity. Following cross-linking, DAF and CD59 become associated in high molecular mass avidin-containing complexes on the membrane. Incorporation of physiological amounts of CD59 into the membranes of biotinylated and avidin cross-linked E is sufficient to render these cells resistant to complement lysis whereas incorporation of DAF has relatively little effect. An understanding of the molecular mechanisms underlying complement susceptibility o...Continue Reading

Citations

Mar 3, 2010·Expert Opinion on Drug Delivery·Vladimir R Muzykantov
Nov 1, 2011·Journal of Microencapsulation·Nadine SternbergHans Bäumler
Jul 24, 2012·Journal of Cellular Biochemistry·Bing LiXian-Ming Chu
Nov 5, 2004·The Journal of Pharmacology and Experimental Therapeutics·Kumkum GangulyJuan Carlos Murciano
Aug 1, 2015·Therapeutic Delivery·Carlos H VillaVladimir R Muzykantov
Jul 8, 2003·Nature Biotechnology·Juan-Carlos MurcianoVladimir R Muzykantov
Jul 10, 2013·International Journal of Oncology·Baijun LiZhiwei Wang
Apr 16, 2020·Advanced Biosystems·Sebastian HimbertMaikel C Rheinstädter
Nov 22, 2005·Expert Opinion on Drug Delivery·Luiga RossiMauro Magnani
Dec 3, 2020·Biosensors & Bioelectronics·Ruixue ZhuIgor Meglinski
Jan 28, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tatsuro OuchiYuichi Ohya
Apr 1, 2021·Annual Review of Biomedical Engineering·Jacob S BrennerVladimir R Muzykantov
Nov 8, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bing LiXianming Chu

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