PMID: 9649632Jul 3, 1998Paper

Enhanced concatemer cloning-a modification to the SAGE (Serial Analysis of Gene Expression) technique

Nucleic Acids Research
J Powell

Abstract

The Serial Analysis of Gene Expression (SAGE) method, described in 1995 by Velculescu et al ., represents a powerful means to compare gene expression between two mRNA populations. An improvement to SAGE that removes contaminating linker molecules, which compromise the efficiency of the method, has been developed. This modification utilises biotinylated PCR primers, which generate biotinylated linkers at an early stage in the SAGE protocol, thus allowing removal of the unwanted linkers by binding to streptavidin-coated magnetic beads at a later stage. The application of this modification resulted in the rapid generation of high ditag yields and clones with large average insert sizes.

References

Oct 20, 1995·Science·V E VelculescuK W Kinzler
Jan 24, 1997·Cell·V E VelculescuK W Kinzler
May 23, 1997·Science·L ZhangK W Kinzler
Aug 28, 1997·Oncogene·S L MaddenG A Beaudry
Sep 26, 1997·Nature·K PolyakB Vogelstein

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Citations

Dec 4, 2003·Mutation Research·Michael D WatersKenneth Olden
Mar 17, 2001·Journal of Immunological Methods·M YamamotoA Ryo
Oct 2, 2001·Pharmacology & Therapeutics·M LeszczynieckaP B Fisher
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Jan 29, 2000·The Plant Journal : for Cell and Molecular Biology·H MatsumuraR Terauchi
Jun 19, 2001·Nucleic Acids Research·E H MarguliesJ W Innis
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Nov 8, 2002·Genome Research·John J DunnMaureen K Krause
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Nov 18, 2003·Immunological Reviews·Stephen P CobboldHerman Waldmann
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Aug 23, 2003·Immunity·Edward J EvansSimon J Davis
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Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kathleen F NolanHerman Waldmann
Mar 3, 2020·Frontiers in Genetics·Xiaonan YangYang Dong
Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole MeissnerMark A Jutila
Jun 2, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·K Polyak, G J Riggins

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