Enhanced Conjugation of Auxin by GH3 Enzymes Leads to Poor Adventitious Rooting in Carnation Stem Cuttings

Frontiers in Plant Science
Antonio CanoJosé Manuel Pérez-Pérez

Abstract

Commercial carnation (Dianthus caryophyllus) cultivars are vegetatively propagated from axillary stem cuttings through adventitious rooting; a process which is affected by complex interactions between nutrient and hormone levels and is strongly genotype-dependent. To deepen our understanding of the regulatory events controlling this process, we performed a comparative study of adventitious root (AR) formation in two carnation cultivars with contrasting rooting performance, "2101-02 MFR" and "2003 R 8", as well as in the reference cultivar "Master". We provided molecular evidence that localized auxin response in the stem cutting base was required for efficient adventitious rooting in this species, which was dynamically established by polar auxin transport from the leaves. In turn, the bad-rooting behavior of the "2003 R 8" cultivar was correlated with enhanced synthesis of indole-3-acetic acid conjugated to aspartic acid by GH3 proteins in the stem cutting base. Treatment of stem cuttings with a competitive inhibitor of GH3 enzyme activity significantly improved rooting of "2003 R 8". Our results allowed us to propose a working model where endogenous auxin homeostasis regulated by GH3 proteins accounts for the cultivar dependenc...Continue Reading

References

Mar 22, 2001·The Plant Journal : for Cell and Molecular Biology·G ParryM J Bennett
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 28, 2002·The Plant Journal : for Cell and Molecular Biology·Hidehiro FukakiMasao Tasaka
Mar 9, 2002·The Plant Cell·Qing TianJason W Reed
Mar 21, 2002·Physiologia Plantarum·Germán GarridoJosé Sánchez-Bravo
Nov 26, 2002·The Plant Journal : for Cell and Molecular Biology·Rosamond G JacksonDianna J Bowles
Jul 29, 2004·The Journal of Biological Chemistry·Kentaro TakeiHitoshi Sakakibara
Jun 3, 2006·The Plant Journal : for Cell and Molecular Biology·Sandra KuuskEva Sundberg
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Aaron M RashotteJoseph J Kieber
Aug 15, 2006·Journal of Plant Physiology·Juana Inés López NicolásJosé Sánchez-Bravo
Oct 18, 2006·Genes & Development·Michael SauerEva Benková
Jan 16, 2007·Plant Physiology·Yuko YamamotoTakashi Sazuka
Sep 2, 2009·Plant Signaling & Behavior·Manuel AcostaJosé Sánchez-Bravo
Mar 1, 2008·Molecular Plant·Ivan A PaponovKlaus Palme
Oct 15, 2009·Molecular Plant·Xiaowei GaoZhenbiao Yang
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ive De SmetTom Beeckman
Mar 3, 2010·Annual Review of Plant Biology·Yunde Zhao
Jul 3, 2010·Journal of Experimental Botany·Martina LankováKlára Hoyerová
Feb 11, 2011·Journal of Experimental Botany·Jutta Ludwig-Müller
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christina WonYunde Zhao
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kiyoshi MashiguchiHiroyuki Kasahara
Mar 27, 2012·Journal of Experimental Botany·Yoshihiro Mano, Keiichirou Nemoto
Apr 25, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Tatsuaki GohHidehiro Fukaki
May 5, 2012·Annual Review of Plant Biology·Ildoo HwangBruno Müller
Jan 29, 2013·Plant Science : an International Journal of Experimental Plant Biology·María Ángeles Agulló-AntónManuel Acosta
May 1, 2013·Journal of Experimental Botany·Edith Pierre-JeromeJennifer L Nemhauser
Oct 15, 2013·Physiologia Plantarum·María Ángeles Agulló-AntónManuel Acosta
Nov 30, 2013·Developmental Cell·Takatoshi KibaHitoshi Sakakibara
Dec 18, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masafumi YagiSatoshi Tabata

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Citations

Oct 14, 2018·International Journal of Molecular Sciences·Vladimír SkalickýOndřej Novák

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Methods Mentioned

BETA
dissection
PCR
reverse transcription PCR
glucosylation

Software Mentioned

StatGraphics Centurion XV
heatmap
SPSS
R
Short Time - Series Expression Miner ( STEM )

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