Enhanced degradation and decreased stability of eye lens alpha-crystallin upon methylglyoxal modification

Experimental Eye Research
M Satish KumarG Bhanuprakash Reddy

Abstract

Methylglyoxal (MGO), a potent glycating agent, forms advanced glycation end products (AGEs) with proteins. Several diabetic complications including cataract are thought to be the result of accumulation of these protein-AGEs. alpha-Crystallin, molecular chaperone of the eye lens, plays an important role in maintaining the transparency of the lens by preventing the aggregation/inactivation of several proteins/enzymes in addition to its structural role. Binding of adenosine-5-triphosphate (ATP) to alpha-crystallin has been shown to enhance its chaperone-like function and protection against proteolytic degradation. In the earlier study, we have shown that modification of alpha-crystallin by MGO caused altered chaperone-like activity along with structural changes, cross-linking, coloration and subsequent insolubilization leading to scattering of light [Biochem. J. 379 (2004) 273]. In the present study, we have investigated ATP binding, stability and degradation of MGO-modified alpha-crystallin. Proteolytic digestion with trypsin and chymotrypsin showed that MGO-modified alpha-crystallin is more susceptible to degradation compared to native alpha-crystallin. Furthermore, ATP was able to protect native alpha-crystallin against proteol...Continue Reading

References

Dec 12, 1989·Biochemistry·B L SteadmanC R Middaugh
Aug 1, 1972·The Biochemical Journal·J J Harding
Jan 25, 1995·Biochimica Et Biophysica Acta·M L Riley, J J Harding
Jan 1, 1993·Molecular Aspects of Medicine·P J Thornalley
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·P J Muchowski, J I Clark
Jul 3, 1998·International Journal of Biological Macromolecules·M Kantorow, J Piatigorsky
Jul 3, 1998·International Journal of Biological Macromolecules·R J TruscottD C Shaw
Jul 3, 1998·International Journal of Biological Macromolecules·L Takemoto, D Boyle
Oct 9, 1999·The Journal of Biological Chemistry·P J MuchowskiJ I Clark
Feb 15, 2000·The Journal of Biological Chemistry·G B ReddyW K Surewicz
Dec 12, 2001·European Journal of Biochemistry·K Wang, A Spector
Apr 9, 2002·Molecular and Cellular Biochemistry·Prajitha ThampiEdathara C Abraham
Jun 7, 2002·The Biochemical Journal·Barry K Derham, John J Harding
Oct 9, 2002·Current Protein & Peptide Science·E Ganea
Feb 5, 2003·Experimental Eye Research·Joseph Horwitz
Sep 10, 2003·Biochemistry·Ram H NagarajAnoop K Padival
Oct 16, 2003·JAMA : the Journal of the American Medical Association·Nathan G CongdonThomas Lietman
Nov 26, 2003·Investigative Ophthalmology & Visual Science·Naila AhmedGeorge M Haik

❮ Previous
Next ❯

Citations

Nov 7, 2008·The British Journal of Nutrition·Megha SaraswatG Bhanuprakash Reddy
Mar 19, 2013·Journal of Proteomics·Ioannis P TrougakosVassilis G Gorgoulis
Jun 7, 2014·Progress in Retinal and Eye Research·Stylianos A KandarakisAthanasios G Papavassiliou
Apr 29, 2015·Expert Review of Proteomics·Evgeny V Mymrikov, Martin Haslbeck
Feb 11, 2016·International Journal of Biological Macromolecules· Jyoti Moinuddin
Mar 28, 2012·The International Journal of Biochemistry & Cell Biology·C GarridoH H Kampinga
Nov 12, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sauradipta BanerjeeAbhay Sankar Chakraborti
May 20, 2015·Biochimica Et Biophysica Acta·Vadde Sudhakar Reddy, G Bhanuprakash Reddy
Mar 1, 2006·The International Journal of Biochemistry & Cell Biology·Jun DuShaohui Cai
Jun 10, 2005·The FEBS Journal·Yu Sun, Thomas H MacRae
Apr 25, 2009·IUBMB Life·Pasupulati Anil Kumar, Geereddy Bhanuprakash Reddy
Aug 8, 2015·Chemical Research in Toxicology·Yingdong ZhuShengmin Sang
Jan 10, 2013·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Haroon AhmadAbdul Wahid
Feb 15, 2015·Journal of Molecular Biology·Martin Haslbeck, Elizabeth Vierling
Sep 16, 2008·The Journal of Nutritional Biochemistry·Pasupulati Anil KumarGeereddy Bhanuprakash Reddy
Dec 1, 2017·Cell Stress & Chaperones·Ivan DimauroDaniela Caporossi
Jun 15, 2005·The Biochemical Journal·M Satish KumarG Bhanuprakash Reddy
Sep 13, 2019·Reproduction in Domestic Animals = Zuchthygiene·Tom SeelingAnne Navarrete Santos
Sep 19, 2017·Biochemistry. Biokhimii︠a︡·M V Sudnitsyna, N B Gusev
Oct 29, 2020·Cell Stress & Chaperones·Daniela CaporossiIvan Dimauro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.