Enhanced degradation of phenol by Pseudomonas sp. CP4 entrapped in agar and calcium alginate beads in batch and continuous processes

Biodegradation
P Y Aneez Ahamad, A A Mohammad Kunhi

Abstract

Phenol is one of the major toxic pollutants in the wastes generated by a number of industries and needs to be eliminated before their discharge. Although microbial degradation is a preferred method of waste treatment for phenol removal, the general inability of the degrading strains to tolerate higher substrate concentrations has been a bottleneck. Immobilization of the microorganism in suitable matrices has been shown to circumvent this problem to some extent. In this study, cells of Pseudomonas sp. CP4, a laboratory isolate that degrades phenol, cresols, and other aromatics, were immobilized by entrapment in Ca-alginate and agar gel beads, separately and their performance in a fluidized bed bioreactor was compared. In batch runs, with an aeration rate of 1 vol(-1) vol(-1) min(-1), at 30°C and pH 7.0 ± 0.2, agar-encapsulated cells degraded up to 3000 mg l(-1) of phenol as compared to 1500 mg l(-1) by Ca-alginate-entrapped cells whereas free cells could tolerate only 1000 mg l(-1). In a continuous process with Ca-alginate entrapped cells a degradation rate of 200 mg phenol l(-1) h(-1) was obtained while agar-entrapped cells were far superior and could withstand and degrade up to 4000 mg phenol l(-1) in the feed with a maximum d...Continue Reading

References

Jul 28, 1999·Applied Microbiology and Biotechnology·K BandhyopadhyayB R Maiti
Aug 31, 2000·Applied and Environmental Microbiology·K WatanabeS Harayama
Jun 8, 2002·Journal of Biotechnology·M Begoña PrietoMaría J Llama
May 23, 2003·Journal of Basic Microbiology·Vera L SantosValter R Linardi
Oct 2, 2003·Bioscience, Biotechnology, and Biochemistry·Wael S El-SayedAkikazu Ando
Jul 7, 2005·Water Research·L AmorM C Veiga
Jul 30, 2005·Journal of Hazardous Materials·Arzu Y Dursun, Ozlem Tepe
Feb 3, 2006·Biodegradation·Chandrakant KarigarDae Jin Yun
Feb 1, 1986·Biotechnology and Bioengineering·A L DaintyM D Trevan
Mar 1, 1995·World Journal of Microbiology & Biotechnology·K S BabuA A Kunhi
Nov 1, 1995·World Journal of Microbiology & Biotechnology·K S BabuA A Kunhi

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Citations

Apr 9, 2011·Applied Microbiology and Biotechnology·Andrew J DaugulisBenoit Guieysse
Jan 25, 2012·Astrobiology·Sara SeagerWilliam Bains
Jul 31, 2012·Chemosphere·Juliana M SaezMaría J Amoroso
Feb 9, 2016·Environmental Technology·Cintia E PaisioElizabeth Agostini
Oct 8, 2016·Microbial Cell Factories·Mercedes Berlanga, Ricardo Guerrero
May 9, 2019·Applied Microbiology and Biotechnology·Maria L Carmona-Orozco, Aram J Panay
Jan 10, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Mengyang TianGuojun Cheng

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